Abstract
A phosphate-modified zirconia was investigated for its potential use as a high-performance inorganic cation-exchange support for the separation of proteins. This phosphate modification effectively blocks the sites responsible for the strong interactions of certain Lewis bases with the zirconia surface. It provides a more "bio-compatible" stationary phase, resulting in high recoveries for proteins and enzymes and retention of their enzymatic activity. The stability, loading capacity, selectivity, efficiency and separation mechanism on the phosphate-modified zirconia are reported. These studies have shown that phosphate-modified zirconia is a useful high-performance ion-exchange support for the separation of cationic proteins and for blocking the sites responsible for the high affinity of zirconia towards certain anions. This makes the phosphate modification interesting in its own right and as an intermediate stage for the development of other zirconia-based chromatographic supports.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 149-160 |
| Number of pages | 12 |
| Journal | Journal of Chromatography A |
| Volume | 587 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 20 1991 |
Bibliographical note
Funding Information:The authors thank Thomas Weber, John Black-well and Dr. Alon McCormick of the University of Minnesota and Dr. Eric Funkenbusch of the 3M Ceramic Technology Center for many helpful discussions and suggestions. W.A.S. also gratefully acknowledges financial support from 3M and the Institute for Advanced Studies at the University of Minnesota.