Chromatin remodeling in nuclear cloning

Paul A. Wade, Nobuaki Kikyo

Research output: Contribution to journalShort surveypeer-review

49 Scopus citations


Nuclear cloning is a procedure to create new animals by injecting somatic nuclei into unfertilized oocytes. Recent successes in mammalian cloning with differentiated adult nuclei strongly indicate that oocyte cytoplasm contains unidentified remarkable reprogramming activities with the capacity to erase the previous memory of cell differentiation. At the heart of this nuclear reprogramming lies chromatin remodeling as chromatin structure and function define cell differentiation through regulation of the transcriptional activities of the cells. Studies involving the modification of chromatin elements such as selective uptake or release of binding proteins, covalent histone modifications including acetylation and methylation, and DNA methylation should provide significant insight into the molecular mechanisms of nuclear dedifferentiation and redifferentiation in oocyte cytoplasm.

Original languageEnglish (US)
Pages (from-to)2284-2287
Number of pages4
JournalEuropean Journal of Biochemistry
Issue number9
StatePublished - 2002


  • Chromatin remodeling
  • DNA methylation
  • Histone acetylation
  • Histone methylation
  • Linker histone
  • Nuclear cloning


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