CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

Theresa W. Chan; Simone Langness; Olga Cohen; Brian P. Eliceiri; Andrew Baird; Todd W. Costantini

doi:10.1007/s00011-020-01349-7

CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

Theresa W. Chan, Simone Langness, Olga Cohen, Brian P. Eliceiri, Andrew Baird, Todd W. Costantini

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Objective and design: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel–cell adhesion, and regulates the expression of genes associated with leukocyte migration. Material: Human THP-1, RAW264.7 and HEK293 cells. Methods: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. Results: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. Conclusion: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

Original language	English (US)
Pages (from-to)	631-633
Number of pages	3
Journal	Inflammation Research
Volume	69
Issue number	7
DOIs	https://doi.org/10.1007/s00011-020-01349-7
State	Published - Jul 1 2020
Externally published	Yes

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Publisher Copyright:
© 2020, Springer Nature Switzerland AG.

Keywords

Dup α7-nicotinic acetylcholine receptor
Human-specific genes
Monocyte migration
Myeloid cell self-renewal
α7-nicotinic acetylcholine receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro",

abstract = "Objective and design: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel–cell adhesion, and regulates the expression of genes associated with leukocyte migration. Material: Human THP-1, RAW264.7 and HEK293 cells. Methods: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. Results: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. Conclusion: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.",

keywords = "Dup α7-nicotinic acetylcholine receptor, Human-specific genes, Monocyte migration, Myeloid cell self-renewal, α7-nicotinic acetylcholine receptor",

author = "Chan, {Theresa W.} and Simone Langness and Olga Cohen and Eliceiri, {Brian P.} and Andrew Baird and Costantini, {Todd W.}",

note = "Publisher Copyright: {\textcopyright} 2020, Springer Nature Switzerland AG.",

year = "2020",

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doi = "10.1007/s00011-020-01349-7",

language = "English (US)",

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T1 - CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

AU - Chan, Theresa W.

AU - Langness, Simone

AU - Cohen, Olga

AU - Eliceiri, Brian P.

AU - Baird, Andrew

AU - Costantini, Todd W.

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Objective and design: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel–cell adhesion, and regulates the expression of genes associated with leukocyte migration. Material: Human THP-1, RAW264.7 and HEK293 cells. Methods: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. Results: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. Conclusion: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

AB - Objective and design: CHRFAM7A is a unique human gene that encodes a dominant negative inhibitor of the α7 nicotinic acetylcholine receptor. We have recently shown that CHRFAM7A is expressed in human leukocytes, increases cel–cell adhesion, and regulates the expression of genes associated with leukocyte migration. Material: Human THP-1, RAW264.7 and HEK293 cells. Methods: Cell migration, cell proliferation and colony formation in soft agar to compare the biological activity of vector vs. CHRFAM7A-transduced cells. Results: We show that gene delivery of CHRFAM7A into the THP-1 human monocytic cell line reduces cell migration, reduces chemotaxis to monocyte chemoattractant protein, and reduces colony formation in soft agar. Conclusion: Taken together, the findings demonstrate that CHRFAM7A regulates the biological activity of monocytes/macrophages to migrate and undergo anchorage-independent growth in vitro.

KW - Dup α7-nicotinic acetylcholine receptor

KW - Human-specific genes

KW - Monocyte migration

KW - Myeloid cell self-renewal

KW - α7-nicotinic acetylcholine receptor

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JO - Inflammation Research

JF - Inflammation Research

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CHRFAM7A reduces monocyte/macrophage migration and colony formation in vitro

Abstract

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Keywords

UN SDGs

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