Pancreatic exocrine function has been demonstrated to be under neuronal regulation. The pathways responsible for this effect, and the long-term consequences of such interactions, are incompletely described. The effects of neuronal depolarization on pancreatic acinar cells were studied to determine whether calcium signaling and c-fos expression were activated. In pancreatic lobules, which contain both neurons and acinar cells, agonists that selectively stimulated neurons increased intracellular calcium in acinar cells. Depolarization also led to the expression c-fos protein in 24% ± 4% of the acinar cells. In AR42J pancreatic acinar cells, cholinergic stimulation demonstrated an average increase of 398 ± 19 nmol/L in intracellular calcium levels, and induced c-fos expression that was time and dose dependent. The data indicate that intrapancreatic neurons induce Ca2+ signaling and early-response gene expression in pancreatic acinar cells.
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Sonicated salmon sperm DNA, trypsin-EGTA (4-\[2-hydroxyethyl\]-l-piperazine ethanesulfonic acid) (HEPES), Tween-20, penicillin/streptomycin solution, formaldehyde, carbachol, atropine, and veratridine were from Sigma Chemical (St. Louis, Mo.). c-los cDNA, chicken gluteraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA, and AR42J cells (passage 17) were purchased from the American Type Culture Collection (Rockville, Md.). Dulbecco's modified Eagle medium, fetal bovine serum, L-glutamine, Supported in part by National Institutes of Health research grant DK43225 and by the Frederick A. C911er Surgical Society Research Fellowship. Presented at the Fortieth Annual Meeting of The Society for Surgery of the Alimentary Tract, Orlando, Fla., May 16-19, 1999 (poster presentation). Reprint requests: Michael W. Mulholland, 2922 Taubman Center, 1500 East Medical Center Dr., Ann Arbor, MI 48109-0331. e-mail: firstname.lastname@example.org
- AR42J cells
- Pancreatic neurotransmission