Cholestan-3β,5α,6β-triol, but not 7-ketocholesterol, suppresses taurocholate-induced mucin secretion by cultured dog gallbladder epithelial cells

Tadashi Yoshida, J. Henriëtte Klinkspoor, Rahul Kuver, Steven P. Wrenn, Eric W. Kaler, Sum P. Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3β,5α,6β-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells. TriolC suppressed mucin secretion by DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)113-118
Number of pages6
JournalFEBS Letters
Volume478
Issue number1-2
DOIs
StatePublished - Jul 28 2000

Keywords

  • Cholesterol
  • Cytotoxicity
  • Epithelial cell
  • Gallbladder
  • Mucin secretion
  • Oxysterol

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