Cholecystokinin-8 induces intracellular calcium signaling in cultured myenteric neurons from neonatal guinea pigs

Weizhen Zhang, Bradley J. Segura, Michael W. Mulholland

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The responsiveness of cultured myenteric neurons to cholecystokinin (CCK-8) was examined using fura-2-based digital microfluorimetric measurement of intracellular calcium ([Ca2+]i). CCK-8 (10-10-10-6M) evoked concentration-dependent increases in percentage of neurons responding (8-52%) and Δ[Ca2+]i (76-169nM). Gastrin (1μM) also induced an increase in [Ca2+]i in 29±6% of neurons (Δ[Ca2+]i: 71±3nM). L-364,718, an antagonist for the CCK-A receptor, blocked [Ca2+]i response to CCK-8. Removal of extracellular calcium eliminated CCK-induced [Ca2+]i increments, as did the addition of the calcium channel inhibitors nickel (1mM) and lanthanum (5mM). Nifedipine (1-50μM) dose-dependently attenuated CCK-caused [Ca2+]i responses. CCK evokes [Ca2+]i signaling in myenteric neurons by the influx of extracellular calcium, likely through L-type calcium channels.

Original languageEnglish (US)
Pages (from-to)1793-1801
Number of pages9
JournalPeptides
Volume23
Issue number10
DOIs
StatePublished - Oct 1 2002
Externally publishedYes

Keywords

  • Calcium channel
  • Enteric nervous system
  • Fura-2 acetoxymethyl ester
  • Myenteric plexus
  • Signal transduction

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