Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models

Rodrigo Delvecchio; Luiza M. Higa; Paula Pezzuto; Ana Luiza Valadão; Patrícia P. Garcez; Fábio L. Monteiro; Erick C. Loiola; André A. Dias; Fábio J.M. Silva; Matthew T. Aliota; Elizabeth A. Caine; Jorge E. Osorio; Maria Bellio; David H. O’Connor; Stevens Rehen; Renato Santana De Aguiar; Andrea Savarino; Loraine Campanati; Amilcar Tanuri

doi:10.3390/v8120322

Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models

Rodrigo Delvecchio, Luiza M. Higa, Paula Pezzuto, Ana Luiza Valadão, Patrícia P. Garcez, Fábio L. Monteiro, Erick C. Loiola, André A. Dias, Fábio J.M. Silva, Matthew T. Aliota, Elizabeth A. Caine, Jorge E. Osorio, Maria Bellio, David H. O’Connor, Stevens Rehen, Renato Santana De Aguiar, Andrea Savarino, Loraine Campanati, Amilcar Tanuri

Research output: Contribution to journal › Article › peer-review

231 Scopus citations

Abstract

Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.

Original language	English (US)
Journal	Viruses
Volume	8
Issue number	12
DOIs	https://doi.org/10.3390/v8120322
State	Published - Dec 1 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.

Keywords

Antiviral
Chloroquine
Microcephaly
Neural stem cell
Zika virus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v8120322

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Delvecchio, R., Higa, L. M., Pezzuto, P., Valadão, A. L., Garcez, P. P., Monteiro, F. L., Loiola, E. C., Dias, A. A., Silva, F. J. M., Aliota, M. T., Caine, E. A., Osorio, J. E., Bellio, M., O’Connor, D. H., Rehen, S., De Aguiar, R. S., Savarino, A., Campanati, L., & Tanuri, A. (2016). Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models. Viruses, 8(12). https://doi.org/10.3390/v8120322

Delvecchio, R, Higa, LM, Pezzuto, P, Valadão, AL, Garcez, PP, Monteiro, FL, Loiola, EC, Dias, AA, Silva, FJM, Aliota, MT, Caine, EA, Osorio, JE, Bellio, M, O’Connor, DH, Rehen, S, De Aguiar, RS, Savarino, A, Campanati, L & Tanuri, A 2016, 'Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models', Viruses, vol. 8, no. 12. https://doi.org/10.3390/v8120322

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abstract = "Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.",

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doi = "10.3390/v8120322",

language = "English (US)",

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AU - Pezzuto, Paula

AU - Valadão, Ana Luiza

AU - Garcez, Patrícia P.

AU - Monteiro, Fábio L.

AU - Loiola, Erick C.

AU - Dias, André A.

AU - Silva, Fábio J.M.

AU - Aliota, Matthew T.

AU - Caine, Elizabeth A.

AU - Osorio, Jorge E.

AU - Bellio, Maria

AU - O’Connor, David H.

AU - Rehen, Stevens

AU - De Aguiar, Renato Santana

AU - Savarino, Andrea

AU - Campanati, Loraine

AU - Tanuri, Amilcar

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.

AB - Zika virus (ZIKV) infection in utero might lead to microcephaly and other congenital defects. Since no specific therapy is available thus far, there is an urgent need for the discovery of agents capable of inhibiting its viral replication and deleterious effects. Chloroquine is widely used as an antimalarial drug, anti-inflammatory agent, and it also shows antiviral activity against several viruses. Here we show that chloroquine exhibits antiviral activity against ZIKV in Vero cells, human brain microvascular endothelial cells, human neural stem cells, and mouse neurospheres. We demonstrate that chloroquine reduces the number of ZIKV-infected cells in vitro, and inhibits virus production and cell death promoted by ZIKV infection without cytotoxic effects. In addition, chloroquine treatment partially reveres morphological changes induced by ZIKV infection in mouse neurospheres.

KW - Antiviral

KW - Chloroquine

KW - Microcephaly

KW - Neural stem cell

KW - Zika virus

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Chloroquine, an endocytosis blocking agent, inhibits zika virus infection in different cell models

Abstract

Bibliographical note

Keywords

UN SDGs

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