Nicotinamide, the amide form of vitamin B3, and budesonide, a synthetic glucocorticoid used in the treatment of asthma, were evaluated to determine their individual and combinational chemopreventive efficacy on benzo(a)pyrene-induced lung tumors in female A/J mice. Nicotinamide fed at a dietary concentration of 0.75% significantly inhibited tumor multiplicity. Nicotinamide by aerosol inhalation at doses up to 15 mg/kg/day did not result in a statistically significant reduction in tumor multiplicity. Finally, dietary nicotinamide was administered with aerosol budesonide and tumor multiplicity reduced by 90% at 1 week and 49% at 8 weeks post last carcinogen dose. We conclude nicotinamide is an effective and safe agent for lung cancer dietary prevention at both early- and late-stage carcinogenesis and that efficacy is increased with aerosol budesonide. Combination chemoprevention with these agents is a well-tolerated and effective strategy which could be clinically advanced to human studies.
Bibliographical noteFunding Information:
We thank Dr. Margie Clapper at Fox Chase Cancer Center for acting as the prime contractor for several of our NIH subcontracts, and Donna M. Jacobson for her longtime administrative support. This work was supported by the NCI Preclinical Contract NO1-CN-43309. Grant No.: HHSN2612004433009C, Work Assignments FCCC 18015-01, 18014-01, 18010-01 (to L.W. Wattenberg).
© 2019 American Association for Cancer Research.
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