Chemical genomic profiling via barcode sequencing to predict compound mode of action

Jeff S. Piotrowski; Scott W. Simpkins; Sheena C. Li; Raamesh Deshpande; Sean J. McIlwain; Irene M. Ong; Chad L. Myers; Charlie Boone; Raymond J. Andersen

doi:10.1007/978-1-4939-2269-7_23

Chemical genomic profiling via barcode sequencing to predict compound mode of action

Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, Raymond J. Andersen

Computer Science and Engineering

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.

Original language	English (US)
Pages (from-to)	299-318
Number of pages	20
Journal	Methods in Molecular Biology
Volume	1263
DOIs	https://doi.org/10.1007/978-1-4939-2269-7_23
State	Published - 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer Science+Business Media New York.

Keywords

Barcode sequencing
Chemical genomics
Functional genomics
Yeast deletion collection

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10.1007/978-1-4939-2269-7_23

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abstract = "Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.",

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AU - Piotrowski, Jeff S.

AU - Simpkins, Scott W.

AU - Li, Sheena C.

AU - Deshpande, Raamesh

AU - McIlwain, Sean J.

AU - Ong, Irene M.

AU - Myers, Chad L.

AU - Boone, Charlie

AU - Andersen, Raymond J.

PY - 2015

Y1 - 2015

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AB - Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.

KW - Barcode sequencing

KW - Chemical genomics

KW - Functional genomics

KW - Yeast deletion collection

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Chemical genomic profiling via barcode sequencing to predict compound mode of action

Abstract

Bibliographical note

Keywords

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