Chemical genomic profiling via barcode sequencing to predict compound mode of action

Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, Raymond J. Andersen

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Chemical genomics is an unbiased, whole-cell approach to characterizing novel compounds to determine mode of action and cellular target. Our version of this technique is built upon barcoded deletion mutants of Saccharomyces cerevisiae and has been adapted to a high-throughput methodology using next-generation sequencing. Here we describe the steps to generate a chemical genomic profile from a compound of interest, and how to use this information to predict molecular mechanism and targets of bioactive compounds.

Original languageEnglish (US)
Pages (from-to)299-318
Number of pages20
JournalMethods in Molecular Biology
Volume1263
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015, Springer Science+Business Media New York.

Keywords

  • Barcode sequencing
  • Chemical genomics
  • Functional genomics
  • Yeast deletion collection

Fingerprint

Dive into the research topics of 'Chemical genomic profiling via barcode sequencing to predict compound mode of action'. Together they form a unique fingerprint.

Cite this