Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents

Birgit Schilling; Christopher J. Collins; Malin M. Young; Xin Guo; Irwin D. Kuntz; Andrew D. Leavitt; Kip Guy; Bradford W. Gibson

Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents

Birgit Schilling
, Christopher J. Collins
, Malin M. Young
, Xin Guo
, Irwin D. Kuntz
, Andrew D. Leavitt
, Kip Guy
, Bradford W. Gibson

Research output: Contribution to conference › Paper › peer-review

Abstract

Chemical cross-linking strategies to determine protein conformation were discussed. Several model proteins such as myoglobin and cytochrome C were reacted with a combinatorial set of amine-specific cross-linking reagents. The set of crosslinkers with different spacers provided different maximum distance constraints between targeted residues. High performance liquid chromatography and mass spectrometry were used to confirm peptide cross-links and to investigate fragmentation pathways.

Original language	English (US)
Pages	125-126
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents. / Schilling, Birgit; Collins, Christopher J.; Young, Malin M. et al.
2002. 125-126 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

@conference{f5fef4c2ec2b43f8959272ab227c54e2,

title = "Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents",

abstract = "Chemical cross-linking strategies to determine protein conformation were discussed. Several model proteins such as myoglobin and cytochrome C were reacted with a combinatorial set of amine-specific cross-linking reagents. The set of crosslinkers with different spacers provided different maximum distance constraints between targeted residues. High performance liquid chromatography and mass spectrometry were used to confirm peptide cross-links and to investigate fragmentation pathways.",

author = "Birgit Schilling and Collins, \{Christopher J.\} and Young, \{Malin M.\} and Xin Guo and Kuntz, \{Irwin D.\} and Leavitt, \{Andrew D.\} and Kip Guy and Gibson, \{Bradford W.\}",

year = "2002",

language = "English (US)",

pages = "125--126",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents

AU - Schilling, Birgit

AU - Collins, Christopher J.

AU - Young, Malin M.

AU - Guo, Xin

AU - Kuntz, Irwin D.

AU - Leavitt, Andrew D.

AU - Guy, Kip

AU - Gibson, Bradford W.

PY - 2002

Y1 - 2002

N2 - Chemical cross-linking strategies to determine protein conformation were discussed. Several model proteins such as myoglobin and cytochrome C were reacted with a combinatorial set of amine-specific cross-linking reagents. The set of crosslinkers with different spacers provided different maximum distance constraints between targeted residues. High performance liquid chromatography and mass spectrometry were used to confirm peptide cross-links and to investigate fragmentation pathways.

AB - Chemical cross-linking strategies to determine protein conformation were discussed. Several model proteins such as myoglobin and cytochrome C were reacted with a combinatorial set of amine-specific cross-linking reagents. The set of crosslinkers with different spacers provided different maximum distance constraints between targeted residues. High performance liquid chromatography and mass spectrometry were used to confirm peptide cross-links and to investigate fragmentation pathways.

UR - https://www.scopus.com/pages/publications/0042246094

UR - https://www.scopus.com/pages/publications/0042246094#tab=citedBy

M3 - Paper

AN - SCOPUS:0042246094

SP - 125

EP - 126

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Chemical cross-linking strategies to determine protein conformation using a combinatorial set of cross-linking reagents

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