TY - JOUR
T1 - Characterizing Viral Infection by Electron Microscopy
T2 - Lessons from the Coronavirus Disease 2019 Pandemic
AU - Akilesh, Shreeram
AU - Nicosia, Roberto F.
AU - Alpers, Charles E.
AU - Tretiakova, Maria
AU - Hsiang, Tien Ying
AU - Gale, Michael
AU - Smith, Kelly D.
N1 - Publisher Copyright:
© 2021 American Society for Investigative Pathology
PY - 2021/2
Y1 - 2021/2
N2 - The severe acute respiratory syndrome coronavirus 2 pandemic has infected millions of individuals in the United States and caused hundreds of thousands of deaths. Direct infection of extrapulmonary tissues has been postulated, and using sensitive techniques, viral RNA has been detected in multiple organs in the body, including the kidney. However, direct infection of tissues outside of the lung has been more challenging to demonstrate. This has been in part due to misinterpretation of electron microscopy studies. In this perspective, we will discuss what is known about coronavirus infection, some of the basic ultrastructural cell biology that has been confused for coronavirus infection of cells, and rigorous criteria that should be used when identifying pathogens by electron microscopy.
AB - The severe acute respiratory syndrome coronavirus 2 pandemic has infected millions of individuals in the United States and caused hundreds of thousands of deaths. Direct infection of extrapulmonary tissues has been postulated, and using sensitive techniques, viral RNA has been detected in multiple organs in the body, including the kidney. However, direct infection of tissues outside of the lung has been more challenging to demonstrate. This has been in part due to misinterpretation of electron microscopy studies. In this perspective, we will discuss what is known about coronavirus infection, some of the basic ultrastructural cell biology that has been confused for coronavirus infection of cells, and rigorous criteria that should be used when identifying pathogens by electron microscopy.
UR - http://www.scopus.com/inward/record.url?scp=85099973155&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099973155&partnerID=8YFLogxK
U2 - 10.1016/j.ajpath.2020.11.003
DO - 10.1016/j.ajpath.2020.11.003
M3 - Review article
C2 - 33227297
AN - SCOPUS:85099973155
SN - 0002-9440
VL - 191
SP - 222
EP - 227
JO - American Journal of Pathology
JF - American Journal of Pathology
IS - 2
ER -
