Characterizing the “sweet spot” for the preservation of a T-cell line using osmolytes

Chia Hsing Pi, Guanglin Yu, Ashley Petersen, Allison Hubel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This study examined the post-thaw recovery of Jurkat cells cryopreserved in single osmolyte solutions containing sucrose, glycerol or isoleucine, as well as in a combination of the three osmolytes. Cell response was determined using low temperature Raman Spectroscopy and variation in post-thaw recovery with composition was analyzed using statistical modeling. Post-thaw recovery of Jurkat cells in single osmolyte was low. A combination of the osmolytes displayed a non-linear relationship between composition and post-thaw recovery, suggesting that interactions exist between the different solutes. The post-thaw recovery for an optimized multicomponent solution was comparable to that observed using 10% dimethyl sulfoxide and a cooling rate of 1 °C/min. Statistical modeling was used to characterize the importance of each osmolyte in the combination and test for interactions between osmolytes. Higher concentrations of glycerol increase post-thaw recovery and interactions between sucrose and glycerol, as well as sucrose and isoleucine improve post-thaw recovery. Raman images clearly demonstrated that damaging intracellular ice formation was observed more often in the presence of single osmolytes as well as non-optimized multi-component solution compositions.

Original languageEnglish (US)
Article number16223
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Glycerol
Sucrose
Jurkat Cells
Isoleucine
T-Lymphocytes
Cell Line
Raman Spectrum Analysis
Ice
Dimethyl Sulfoxide
Temperature

Cite this

Characterizing the “sweet spot” for the preservation of a T-cell line using osmolytes. / Pi, Chia Hsing; Yu, Guanglin; Petersen, Ashley; Hubel, Allison.

In: Scientific reports, Vol. 8, No. 1, 16223, 01.12.2018.

Research output: Contribution to journalArticle

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