Synchrotron small-angle X-ray scattering analysis of the bilayer structure of a pharmacologically relevant sterically stabilized liposome system is presented. Describing the electron density profile of the bilayer with the superposition of Gaussian functions, the contribution of the poly(ethylene glycol) (PEG) layers to the total electron density was identified. The changes in the thickness of the PEG layer as well as the distribution of the PEG chains among the outer and inner leaflets of the bilayers were followed by changing the molar ratio of the PEG-lipid and the molar weight of the PEG molecule.
Bibliographical noteFunding Information:
This work was supported by the Hungarian Scientific Research Fund (OTKA, Hungary) and the National Innovation Office (NIH, Hungary) under grant agreement CNK-81056 . The research leading to these results has received funding from the European Community's Seventh Framework Programme (FP7/2007–2013) under grant agreement no 226716 at DESY/HASYLAB. We are grateful to the European Synchrotron Radiation Facility for provision of beam time at ID02 (proposal no. SC-3269).
- Poly(ethylene glycol)
- Small-angle X-ray scattering
- Sterical stabilization
- Structural characterization