Characterization of the mouse Muc3 membrane bound intestinal mucin 5′ coding and promoter regions: Regulation by inflammatory cytokines

Laurie L. Shekels, Samuel B. Ho

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The mouse Muc3 mucin is a membrane-bound glycoprotein highly expressed in the intestinal tract. We have characterized the mouse Muc3 5′ structure and regulation of its promoter by cytokines and growth factors. The first two exons of Muc3 are separated by an intron of over 8 kb. Exon 3 contains the tandem repeat domain. Ten exons reside 3′ to the tandem repeat domain. The 5′ nonrepetitive sequence contains 104 amino acids characterized by a putative signal sequence, a single cysteine and 28% serine/threonine. No TATA box is found near the transcription start site. The promoter has consensus binding sites for AP1, CREB, SP1, NFκB, GATA binding protein and Cdx. Muc3 promoter constructs demonstrate that IL4, IL6, EGF or PMA increased promoter activity to 35-58% of control. TNFα and IFNγ showed lesser stimulation. These data indicate that cytokines and growth factors are capable of regulating Muc3 gene expression, suggesting that this protein may play an active role in intestinal mucosal defense.

Original languageEnglish (US)
Pages (from-to)90-100
Number of pages11
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1627
Issue number2-3
DOIs
StatePublished - Jun 19 2003

Keywords

  • Cytokine
  • Gene regulation
  • Glycoprotein
  • Intestine
  • Muc3
  • Mucin

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