The increasing prevalence of multidrug-resistant (MDR) Salmonella is a serious public health threat. Intervention strategies available to control Salmonella mostly target Salmonella enterica serovars Typhimurium and Enteritidis, and little has been investigated to control serovars in serogroup C, such as S. enterica serovar Thompson, despite their increasing prevalence. Here, we isolated phages targeting MDR S. Thompson and characterized the antimicrobial activities of MSP1 phage, a virulent phage with a broad host range. MSP1 phage strongly infected S. Thompson and S. Mbandaka isolates from retail chicken and also other serovars, including Dublin, Enteritidis, Heidelberg, Paratyphi, and Typhimurium. MSP1 phage was able to inhibit the biofilm formation on stainless steel and glass formation by around 42.7–47.9 %. MSP1 phage was robust to withstand wide ranges of pH (4–12) and temperature (30–60 °C), and no genes associated with antibiotic resistance and virulence were found in the phage genome, suggesting that this phage is suitable for food application. When MSP1 phage was tested on foods (chicken meat and milk), MSP1 phage significantly reduced the level of MDR S. Thompson below the detection limit. Our findings suggest that MSP1 phage is a promising antimicrobial agent for the control of food contamination by MDR S. Thompson.
Bibliographical noteFunding Information:
This research was supported by Basic Science Research Programs ( 2020R1A2B5B03094303 ) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning and a grant 20162MFDS142 from Ministry of Food and Drug Safety in 2021. J.K. was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education ( 2022R1A6A1A03055869 ).
© 2022 Elsevier B.V.
- Multidrug-resistant Salmonella
- Processing facility
PubMed: MeSH publication types
- Journal Article