Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

David A. Mills; Trevor G. Phister; Kathleen A. Baldwin; Gary M Dunny; Larry L. McKay

doi:10.1023/A:1009759525576

Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

David A. Mills
, Trevor G. Phister
, Kathleen A. Baldwin
, Gary M Dunny
, Larry L. McKay

Microbiology

Research output: Contribution to journal › Article › peer-review

Abstract

Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.

Original language	English (US)
Pages (from-to)	71-78
Number of pages	8
Journal	Methods in Cell Science
Volume	20
Issue number	1-4
DOIs	https://doi.org/10.1023/A:1009759525576
State	Published - 1998

Bibliographical note

Funding Information:
This work was supported by grants from the Minnesota-South Dakota Dairy Foods Research Center (G.M.D.) and the Kraft General Foods Chair (L.L.M.). D.A.M. was supported by a NIGMS Predoctoral Biotechnology Training Grant.

Keywords

Conjugation
Lactic acid bacteria
Lactococci
Mobilization
oriT

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10.1023/A:1009759525576

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abstract = "Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.",

keywords = "Conjugation, Lactic acid bacteria, Lactococci, Mobilization, oriT",

author = "Mills, \{David A.\} and Phister, \{Trevor G.\} and Baldwin, \{Kathleen A.\} and Dunny, \{Gary M\} and McKay, \{Larry L.\}",

note = "Funding Information: This work was supported by grants from the Minnesota-South Dakota Dairy Foods Research Center (G.M.D.) and the Kraft General Foods Chair (L.L.M.). D.A.M. was supported by a NIGMS Predoctoral Biotechnology Training Grant.",

year = "1998",

doi = "10.1023/A:1009759525576",

language = "English (US)",

volume = "20",

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T1 - Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

AU - Mills, David A.

AU - Phister, Trevor G.

AU - Baldwin, Kathleen A.

AU - Dunny, Gary M

AU - McKay, Larry L.

N1 - Funding Information: This work was supported by grants from the Minnesota-South Dakota Dairy Foods Research Center (G.M.D.) and the Kraft General Foods Chair (L.L.M.). D.A.M. was supported by a NIGMS Predoctoral Biotechnology Training Grant.

PY - 1998

Y1 - 1998

N2 - Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.

AB - Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.

KW - Conjugation

KW - Lactic acid bacteria

KW - Lactococci

KW - Mobilization

KW - oriT

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M3 - Article

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JO - Methods in Cell Science

JF - Methods in Cell Science

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ER -

Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

Abstract

Bibliographical note

Keywords

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