Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis

David A. Mills, Trevor G. Phister, Kathleen A. Baldwin, Gary M Dunny, Larry L. McKay

Research output: Contribution to journalArticlepeer-review


Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.

Original languageEnglish (US)
Pages (from-to)71-78
Number of pages8
JournalMethods in Cell Science
Issue number1-4
StatePublished - 1998

Bibliographical note

Funding Information:
This work was supported by grants from the Minnesota-South Dakota Dairy Foods Research Center (G.M.D.) and the Kraft General Foods Chair (L.L.M.). D.A.M. was supported by a NIGMS Predoctoral Biotechnology Training Grant.


  • Conjugation
  • Lactic acid bacteria
  • Lactococci
  • Mobilization
  • oriT


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