Conjugation is a common mode of genetic transfer among the lactic acid bacteria. In an effort to exploit conjugation as a means of lactococcal strain development, we have characterized the transfer regions of pRS01 by insertional mutagenesis via IS946-mediated cointegration with the lactococcal-Escherichia coli shuttle plasmid pTRK28. In this work we will discuss our use of pTRK28-mediated cointegration to identify, clone, and characterize the pRS01 transfer regions including the conjugative origin of transfer.
Bibliographical noteFunding Information:
This work was supported by grants from the Minnesota-South Dakota Dairy Foods Research Center (G.M.D.) and the Kraft General Foods Chair (L.L.M.). D.A.M. was supported by a NIGMS Predoctoral Biotechnology Training Grant.
- Lactic acid bacteria