Characterization of the calcium-binding sites of calcineurin B

Lazaros T. Kakalis; Michael Kennedy; Robert Sikkink; Frank Rusnak; Ian M. Armitage

doi:10.1016/0014-5793(95)00207-P

Characterization of the calcium-binding sites of calcineurin B

Lazaros T. Kakalis, Michael Kennedy, Robert Sikkink, Frank Rusnak, Ian M. Armitage

Biochemistry, Molecular Biology, and Biophysics (TMED)

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Calcineurin (CaN) is a calcium- and calmodulin-dependent serine/threonine phosphatase whose inhibition by the immunosuppressant-immunophilin complexes (cyclosporin-cyclophilin and FK506-FKBP) is considered key to the mechanism of immunosuppression. CaN is a heterodimer, consisting of a 59 kDa catalytic subunit (A) and a 19 kDa calcium-binding regulatory subunit (B). The latter is postulated to harbor four calcium binding domains of the EF hand type. The titration of the CaN B apoprotein with the isomorphic Cd²⁺ was followed by ¹¹³Cd NMR and these data support one high-affinity metal binding site and three lower-affinity ones. Flow dialysis data with Ca²⁺ indicate one high affinity calcium binding site with K_d ∼ 2.4 × 10^-8 M and three other sites with K_d ∼ 1.5 × 10^-5 M. The chemical shifts of all four ¹¹³Cd resonances (-75, -93, -106 and -119 ppm) are in the same range as found in other ¹¹³Cd substituted calcium-binding proteins, and are indicative of all-oxygen coordination of pentagonal bipyramidal geometry.

Original language	English (US)
Pages (from-to)	55-58
Number of pages	4
Journal	FEBS Letters
Volume	362
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00207-P
State	Published - Mar 27 1995

Bibliographical note

Funding Information:
Acknowledgements." This study was supported by grants from NIH (GM49858 to I.M.A. and GM46865 to F.R.). NMR instrumentation and computational facilities were provided by grants from NIH (RR03475), NSF (DMB8610557), and ACS (RD259). Access to the atomic absorption spectrophotometer at the Clinical Chemistry Laboratory of the Yale-New Haven Hospital is gratefully acknowledged.

Keywords

Cadmium-113 NMR
Calcineurin
Calcium binding
EF hand

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title = "Characterization of the calcium-binding sites of calcineurin B",

abstract = "Calcineurin (CaN) is a calcium- and calmodulin-dependent serine/threonine phosphatase whose inhibition by the immunosuppressant-immunophilin complexes (cyclosporin-cyclophilin and FK506-FKBP) is considered key to the mechanism of immunosuppression. CaN is a heterodimer, consisting of a 59 kDa catalytic subunit (A) and a 19 kDa calcium-binding regulatory subunit (B). The latter is postulated to harbor four calcium binding domains of the EF hand type. The titration of the CaN B apoprotein with the isomorphic Cd2+ was followed by 113Cd NMR and these data support one high-affinity metal binding site and three lower-affinity ones. Flow dialysis data with Ca2+ indicate one high affinity calcium binding site with Kd ∼ 2.4 × 10-8 M and three other sites with Kd ∼ 1.5 × 10-5 M. The chemical shifts of all four 113Cd resonances (-75, -93, -106 and -119 ppm) are in the same range as found in other 113Cd substituted calcium-binding proteins, and are indicative of all-oxygen coordination of pentagonal bipyramidal geometry.",

keywords = "Cadmium-113 NMR, Calcineurin, Calcium binding, EF hand",

author = "Kakalis, {Lazaros T.} and Michael Kennedy and Robert Sikkink and Frank Rusnak and Armitage, {Ian M.}",

note = "Funding Information: Acknowledgements.{"} This study was supported by grants from NIH (GM49858 to I.M.A. and GM46865 to F.R.). NMR instrumentation and computational facilities were provided by grants from NIH (RR03475), NSF (DMB8610557), and ACS (RD259). Access to the atomic absorption spectrophotometer at the Clinical Chemistry Laboratory of the Yale-New Haven Hospital is gratefully acknowledged.",

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AU - Kakalis, Lazaros T.

AU - Kennedy, Michael

AU - Sikkink, Robert

AU - Rusnak, Frank

AU - Armitage, Ian M.

N1 - Funding Information: Acknowledgements." This study was supported by grants from NIH (GM49858 to I.M.A. and GM46865 to F.R.). NMR instrumentation and computational facilities were provided by grants from NIH (RR03475), NSF (DMB8610557), and ACS (RD259). Access to the atomic absorption spectrophotometer at the Clinical Chemistry Laboratory of the Yale-New Haven Hospital is gratefully acknowledged.

PY - 1995/3/27

Y1 - 1995/3/27

N2 - Calcineurin (CaN) is a calcium- and calmodulin-dependent serine/threonine phosphatase whose inhibition by the immunosuppressant-immunophilin complexes (cyclosporin-cyclophilin and FK506-FKBP) is considered key to the mechanism of immunosuppression. CaN is a heterodimer, consisting of a 59 kDa catalytic subunit (A) and a 19 kDa calcium-binding regulatory subunit (B). The latter is postulated to harbor four calcium binding domains of the EF hand type. The titration of the CaN B apoprotein with the isomorphic Cd2+ was followed by 113Cd NMR and these data support one high-affinity metal binding site and three lower-affinity ones. Flow dialysis data with Ca2+ indicate one high affinity calcium binding site with Kd ∼ 2.4 × 10-8 M and three other sites with Kd ∼ 1.5 × 10-5 M. The chemical shifts of all four 113Cd resonances (-75, -93, -106 and -119 ppm) are in the same range as found in other 113Cd substituted calcium-binding proteins, and are indicative of all-oxygen coordination of pentagonal bipyramidal geometry.

AB - Calcineurin (CaN) is a calcium- and calmodulin-dependent serine/threonine phosphatase whose inhibition by the immunosuppressant-immunophilin complexes (cyclosporin-cyclophilin and FK506-FKBP) is considered key to the mechanism of immunosuppression. CaN is a heterodimer, consisting of a 59 kDa catalytic subunit (A) and a 19 kDa calcium-binding regulatory subunit (B). The latter is postulated to harbor four calcium binding domains of the EF hand type. The titration of the CaN B apoprotein with the isomorphic Cd2+ was followed by 113Cd NMR and these data support one high-affinity metal binding site and three lower-affinity ones. Flow dialysis data with Ca2+ indicate one high affinity calcium binding site with Kd ∼ 2.4 × 10-8 M and three other sites with Kd ∼ 1.5 × 10-5 M. The chemical shifts of all four 113Cd resonances (-75, -93, -106 and -119 ppm) are in the same range as found in other 113Cd substituted calcium-binding proteins, and are indicative of all-oxygen coordination of pentagonal bipyramidal geometry.

KW - Cadmium-113 NMR

KW - Calcineurin

KW - Calcium binding

KW - EF hand

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Characterization of the calcium-binding sites of calcineurin B

Abstract

Bibliographical note

Keywords

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