Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine

David R. Brown, Lisa D. Price

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.

Original languageEnglish (US)
Pages (from-to)328-333
Number of pages6
JournalVeterinary Microbiology
Volume120
Issue number3-4
DOIs
StatePublished - Mar 10 2007

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health grant R01 DA-10200.

Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.

Keywords

  • Actin
  • Intestinal epithelium
  • Neural Wiskott-Aldrich syndrome protein
  • Rho GTPase
  • Time course

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