Abstract
Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 328-333 |
| Number of pages | 6 |
| Journal | Veterinary Microbiology |
| Volume | 120 |
| Issue number | 3-4 |
| DOIs | |
| State | Published - Mar 10 2007 |
Bibliographical note
Funding Information:This work was supported in part by National Institutes of Health grant R01 DA-10200.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
Keywords
- Actin
- Intestinal epithelium
- Neural Wiskott-Aldrich syndrome protein
- Rho GTPase
- Time course