Characterization of S class gene segments of a newly isolated turkey arthritis reovirus

Sunil K. Mor; Harsha Verma; Tamer A. Sharafeldin; Robert E. Porter; Naresh Jindal; Andre Ziegler; Sagar M. Goyal

doi:10.1016/j.virol.2014.06.009

Characterization of S class gene segments of a newly isolated turkey arthritis reovirus

Sunil K. Mor, Harsha Verma, Tamer A. Sharafeldin, Robert E. Porter, Naresh Jindal, Andre Ziegler, Sagar M. Goyal

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11 Scopus citations

Abstract

We report on the complete characterization of S class gene segments of 12 newly isolated turkey arthritis reoviruses (TARVs) and compare it with that of a turkey enteric reovirus (TERV). Phylogenetic analysis of S2, S3 and S4 genome segments revealed grouping of all TARVs into two lineages while, on the basis of S1 genome segment, only one lineage was found. All TARVs had 95-100% nucleotide identity based on sigma C protein sequences (S1 segment) but varied from 90-100%, 88.9-100% and 88.7-100% on the basis of S2, S3, and S4 genome segments, respectively. Point mutations as well as possible re-assortments were observed in TARVs throughout the S class indicating the need for extensive epidemiological studies on these viruses in hatcheries and commercial farms, which would be useful in determining virus variation in the field.

Original language	English (US)
Pages (from-to)	33-44
Number of pages	12
Journal	Virology
Volume	464-465
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2014.06.009
State	Published - Sep 2014

Bibliographical note

Funding Information:
This study was funded in part by a grant from the Rapid Agricultural Response Fund, University of Minnesota . We thank Douglas Marthaler for helpful discussions. We also thank Wendy Wiese and Jonathan Erber for technical assistance.

Keywords

Avian reovirus
Phylogenetic analysis
S class gene segments
Turkey arthritis reovirus
Turkey enteric reovirus

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10.1016/j.virol.2014.06.009

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title = "Characterization of S class gene segments of a newly isolated turkey arthritis reovirus",

abstract = "We report on the complete characterization of S class gene segments of 12 newly isolated turkey arthritis reoviruses (TARVs) and compare it with that of a turkey enteric reovirus (TERV). Phylogenetic analysis of S2, S3 and S4 genome segments revealed grouping of all TARVs into two lineages while, on the basis of S1 genome segment, only one lineage was found. All TARVs had 95-100% nucleotide identity based on sigma C protein sequences (S1 segment) but varied from 90-100%, 88.9-100% and 88.7-100% on the basis of S2, S3, and S4 genome segments, respectively. Point mutations as well as possible re-assortments were observed in TARVs throughout the S class indicating the need for extensive epidemiological studies on these viruses in hatcheries and commercial farms, which would be useful in determining virus variation in the field.",

keywords = "Avian reovirus, Phylogenetic analysis, S class gene segments, Turkey arthritis reovirus, Turkey enteric reovirus",

author = "Mor, {Sunil K.} and Harsha Verma and Sharafeldin, {Tamer A.} and Porter, {Robert E.} and Naresh Jindal and Andre Ziegler and Goyal, {Sagar M.}",

note = "Funding Information: This study was funded in part by a grant from the Rapid Agricultural Response Fund, University of Minnesota . We thank Douglas Marthaler for helpful discussions. We also thank Wendy Wiese and Jonathan Erber for technical assistance. ",

year = "2014",

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doi = "10.1016/j.virol.2014.06.009",

language = "English (US)",

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AU - Mor, Sunil K.

AU - Verma, Harsha

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AU - Porter, Robert E.

AU - Jindal, Naresh

AU - Ziegler, Andre

AU - Goyal, Sagar M.

N1 - Funding Information: This study was funded in part by a grant from the Rapid Agricultural Response Fund, University of Minnesota . We thank Douglas Marthaler for helpful discussions. We also thank Wendy Wiese and Jonathan Erber for technical assistance.

PY - 2014/9

Y1 - 2014/9

N2 - We report on the complete characterization of S class gene segments of 12 newly isolated turkey arthritis reoviruses (TARVs) and compare it with that of a turkey enteric reovirus (TERV). Phylogenetic analysis of S2, S3 and S4 genome segments revealed grouping of all TARVs into two lineages while, on the basis of S1 genome segment, only one lineage was found. All TARVs had 95-100% nucleotide identity based on sigma C protein sequences (S1 segment) but varied from 90-100%, 88.9-100% and 88.7-100% on the basis of S2, S3, and S4 genome segments, respectively. Point mutations as well as possible re-assortments were observed in TARVs throughout the S class indicating the need for extensive epidemiological studies on these viruses in hatcheries and commercial farms, which would be useful in determining virus variation in the field.

AB - We report on the complete characterization of S class gene segments of 12 newly isolated turkey arthritis reoviruses (TARVs) and compare it with that of a turkey enteric reovirus (TERV). Phylogenetic analysis of S2, S3 and S4 genome segments revealed grouping of all TARVs into two lineages while, on the basis of S1 genome segment, only one lineage was found. All TARVs had 95-100% nucleotide identity based on sigma C protein sequences (S1 segment) but varied from 90-100%, 88.9-100% and 88.7-100% on the basis of S2, S3, and S4 genome segments, respectively. Point mutations as well as possible re-assortments were observed in TARVs throughout the S class indicating the need for extensive epidemiological studies on these viruses in hatcheries and commercial farms, which would be useful in determining virus variation in the field.

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KW - Phylogenetic analysis

KW - S class gene segments

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KW - Turkey enteric reovirus

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Characterization of S class gene segments of a newly isolated turkey arthritis reovirus

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