Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line

Guney Boso, Nikunj V. Somia

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1 Scopus citations


Viruses interact with various permissive and restrictive factors in host cells throughout their replication cycle. Cell lines that are non-permissive to viral infection have been particularly useful in discovering host cell proteins involved in viral life cycles. Here we describe the characterization of a human myeloid leukemia cell line, KG-1, that is resistant to infection by retroviruses and a Rhabdovirus. We show that KG-1 cells are resistant to infection by Vesicular Stomatits Virus as well as VSV Glycoprotein (VSVG) pseudotyped retroviruses due to a defect in binding. Moreover our results indicate that entry by xenotropic retroviral envelope glycoprotein RD114 is impaired in KG-1 cells. Finally we characterize a post- entry block in the early phase of the retroviral life cycle in KG-1 cells that renders the cell line refractory to infection. This cell line will have utility in discovering proteins involved in infection by VSV and HIV-1.

Original languageEnglish (US)
Article numbere0121455
JournalPloS one
Issue number3
StatePublished - Mar 26 2015

Bibliographical note

Funding Information:
We would like to acknowledge the Flow Cytometry Core Facility of the University of Minnesota Cancer Center, a comprehensive cancer center designated by the National Cancer Institute, supported in part by P30 CA77598. We also thank Drs. Bruce Chesebro and Kathy Wehrly for a providing p24 Monoclonal Antibody (183-H12-5C) and Dr. Nathaniel Landau for the plasmid pSV-Ψ-MLV-env that were provided by the AIDS Research and Reference Reagent Program. VSV-eGFP was a kind gift from Dr. Asit Pattnaik (University of Nebraska-Lincoln) and Measles-GFP was a kind gift from Dr. Stephen Russell (Mayo Clinic, Rochester).

Publisher Copyright:
© 2015 Boso, Somia.


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