Abstract
Viruses interact with various permissive and restrictive factors in host cells throughout their replication cycle. Cell lines that are non-permissive to viral infection have been particularly useful in discovering host cell proteins involved in viral life cycles. Here we describe the characterization of a human myeloid leukemia cell line, KG-1, that is resistant to infection by retroviruses and a Rhabdovirus. We show that KG-1 cells are resistant to infection by Vesicular Stomatits Virus as well as VSV Glycoprotein (VSVG) pseudotyped retroviruses due to a defect in binding. Moreover our results indicate that entry by xenotropic retroviral envelope glycoprotein RD114 is impaired in KG-1 cells. Finally we characterize a post- entry block in the early phase of the retroviral life cycle in KG-1 cells that renders the cell line refractory to infection. This cell line will have utility in discovering proteins involved in infection by VSV and HIV-1.
| Original language | English (US) |
|---|---|
| Article number | e0121455 |
| Journal | PloS one |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 26 2015 |
Bibliographical note
Funding Information:We would like to acknowledge the Flow Cytometry Core Facility of the University of Minnesota Cancer Center, a comprehensive cancer center designated by the National Cancer Institute, supported in part by P30 CA77598. We also thank Drs. Bruce Chesebro and Kathy Wehrly for a providing p24 Monoclonal Antibody (183-H12-5C) and Dr. Nathaniel Landau for the plasmid pSV-Ψ-MLV-env that were provided by the AIDS Research and Reference Reagent Program. VSV-eGFP was a kind gift from Dr. Asit Pattnaik (University of Nebraska-Lincoln) and Measles-GFP was a kind gift from Dr. Stephen Russell (Mayo Clinic, Rochester).
Publisher Copyright:
© 2015 Boso, Somia.
