Characterization of nanofibers formed by self-assembly of Β-peptide oligomers using small angle x-ray scattering

Claire L. Pizzey, William C. Pomerantz, Bong June Sung, Virany M. Yuwono, Samuel H. Gellman, Jeffery D. Hartgerink, Arun Yethiraj, Nicholas L. Abbott

Research output: Contribution to journalArticle

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Abstract

Helical oligomers of Β -peptides represent a particularly promising type of building block for directed assembly of organic nanostructures because the helical secondary structure can be designed to be very stable and because control of the Β -amino acid sequence can lead to precise patterning of chemical functional groups over the helix surfaces. In this paper, we report the use of small angle x-ray scattering measurements (SAXS) to characterize nanostructures formed by the directed assembly of Β -peptide A with sequence H2 N- Β3 hTyr- Β3 hLys- Β3 hPhe-ACHC- Β3 hPhe-ACHC- Β3 hPhe- Β3 hLys-ACHC-ACHC- Β3 hPhe- Β3 hLys-CON H2. Whereas prior cryo-TEM studies have revealed the presence of nanofibers in aqueous solutions of Β -peptide A, SAXS measurements from the nanofibers were not well-fit by a form factor model describing solid nanofibers. An improved fit to the scattering data at high q was obtained by using a form factor model describing a cylinder with a hollow center and radial polydispersity. When combined with a structure factor calculated from the polymer reference interaction site model (PRISM) theory, the scattered intensity of x-rays measured over the entire q range was well described by the model. Analysis of our SAXS data suggests a model in which individual Β -peptides assemble to form long cylindrical nanofibers with a hollow core radius of 15 Å (polydispersity of 21%) and a shell thickness of 20 Å. This model is supported by negative stain transmission electron microscopy.

Original languageEnglish (US)
Article number095103
JournalJournal of Chemical Physics
Volume129
Issue number9
DOIs
StatePublished - Sep 15 2008

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x ray scattering
Nanofibers
oligomers
Oligomers
Self assembly
peptides
self assembly
Scattering
X rays
Peptides
Polydispersity
form factors
hollow
Nanostructures
assembly
Transmission electron microscopy
transmission electron microscopy
helices
Functional groups
amino acids

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Characterization of nanofibers formed by self-assembly of Β-peptide oligomers using small angle x-ray scattering. / Pizzey, Claire L.; Pomerantz, William C.; Sung, Bong June; Yuwono, Virany M.; Gellman, Samuel H.; Hartgerink, Jeffery D.; Yethiraj, Arun; Abbott, Nicholas L.

In: Journal of Chemical Physics, Vol. 129, No. 9, 095103, 15.09.2008.

Research output: Contribution to journalArticle

Pizzey, Claire L. ; Pomerantz, William C. ; Sung, Bong June ; Yuwono, Virany M. ; Gellman, Samuel H. ; Hartgerink, Jeffery D. ; Yethiraj, Arun ; Abbott, Nicholas L. / Characterization of nanofibers formed by self-assembly of Β-peptide oligomers using small angle x-ray scattering. In: Journal of Chemical Physics. 2008 ; Vol. 129, No. 9.
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