Characterization of Miniature Probes for Cryosurgery, Thermal Ablation, and Irreversible Electroporation on Small Animals

Pegah Ranjbartehrani, Michael Etheridge, Satish Ramadhyani, Harishankar Natesan, John Bischof, Qi Shao

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Focal therapies, including cryosurgery, thermal ablation, and irreversible electroporation (IRE) are widely used in the treatment of cancer and other diseases, aimed at exposing cancer cells to extreme physical conditions leading to cell death. Focal ablation outcomes are extensively studied in small animal preclinical models, such as mice; however, robust engineering characterization of these treatments is largely lacking, due in a large part to the lack of control in applying clinical devices (cm scale) to mouse tumors (mm scale). To address this issue, miniaturized probes are designed and built for controlled application of cryosurgery, thermal ablation, and IRE and their performance is evaluated using ex vivo porcine liver tissue and an in vivo MC-38 tumor model. These experimental results are compared to simulated conditions in heat transfer (cryosurgery and thermal ablation) and electrical (IRE) models. Agreements of physical quantities between measurement and modeling are shown for all the focal therapy cases. The in vivo outcome is further correlated with tumor growth delay. The characterization improves the understanding of the energy dose to create tissue damage, therefore enables to better understand outcomes in preclinical studies and predict and optimize the outcome of future clinical procedures.

Original languageEnglish (US)
Article number2100212
JournalAdvanced Therapeutics
Volume5
Issue number5
DOIs
StatePublished - May 2022

Bibliographical note

Funding Information:
Computational work was conducted at the University of Minnesota Supercomputing Institute. Porcine livers were donated from animals used in other studies from Visible Heart Lab (PI Paul Iaizzo) at the University of Minnesota, where handling and treatment animals were in compliance of approved IACUC protocols. This work was funded discovery funds from Boston Scientific Corporation (Maple Grove, MN), discovery funds from BTG plc. (Farnham, United Kingdom), and the Dr. Ralph and Marian Falk Medical Research Trust Bank of America, N.A.

Funding Information:
Computational work was conducted at the University of Minnesota Supercomputing Institute. Porcine livers were donated from animals used in other studies from Visible Heart Lab (PI Paul Iaizzo) at the University of Minnesota, where handling and treatment animals were in compliance of approved IACUC protocols. This work was funded discovery funds from Boston Scientific Corporation (Maple Grove, MN), discovery funds from BTG plc. (Farnham, United Kingdom), and the Dr. Ralph and Marian Falk Medical Research Trust Bank of America, N.A.

Publisher Copyright:
© 2022 The Authors. Advanced Therapeutics published by Wiley-VCH GmbH.

Keywords

  • cancer
  • cryosurgery
  • device characterization
  • focal therapy
  • irreversible electroporation
  • modeling
  • simulation
  • temperature
  • thermal ablation
  • tumor

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