Characterization of Invasion of Genus Aspergillus on Peanut Seeds Using FTIR-PAS

Hande Kaya-Celiker, Kumar Mallikarjunan, Archileo Kaaya

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aspergillus colonization on peanuts is a growing concern, as it results in reduced crop yields or livestock productivity due to consumption of contaminated feed, and most importantly, they are famous as causative agents of opportunistic infestation in man. In order to defeat and evaluate peanuts suffering from Aspergillus infection, it is essential to establish proper technique and track the quality of peanuts at both farm and market levels. This study focuses on usage of Fourier transform mid-infrared spectroscopy (FTIR) with a non-invasive reflectance apparatus, photoacoustic spectroscopy (PAS) to identify and separate infected peanuts based on spectral characteristics of infrared radiation on peanuts. Classes were defined as “clean” representing no-aflatoxin/no-mold in peanuts, “moldy” representing peanuts with non-aflatoxigenic Aspergillus strains, and lastly, “toxic” representing peanuts infected with aflatoxigenic strains of Aspergillus spp. Classes were analyzed using discriminant analysis algorithm, and distance values were calculated in Mahalanobis distance units. The spectral ranges between 3600 and 2750, 1800 and 1480, and 1200 and 900 cm−1 were assigned as the key bands, and corresponding vibration modes and intensities were labeled. All healthy, clean peanuts (15 healthy/69 total peanut pods) were successfully separated from moldy ones. Separation was further detailed to distinguish peanuts infected with aflatoxin producing strains of Aspergillus flavus and Aspergillus parasiticus, and as a result, 87 % of samples were separated correctly as only moldy or toxic streams. Performance test was conducted with 36 different samples including toxic, moldy, and clean samples; 80.6 % correct classification was achieved.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalFood Analytical Methods
Volume9
Issue number1
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

photoacoustic spectroscopy
Photoacoustic spectroscopy
Aspergillus
infrared spectroscopy
Fourier Transform Infrared Spectroscopy
invasion
Fourier transform infrared spectroscopy
peanuts
Seed
Spectrum Analysis
Seeds
Poisons
seeds
Aflatoxins
discriminant analysis
colonization
Farms
farm
productivity
market

Keywords

  • Aspergillus species
  • Discriminant analysis
  • FTIR-PAS
  • Peanut

Cite this

Characterization of Invasion of Genus Aspergillus on Peanut Seeds Using FTIR-PAS. / Kaya-Celiker, Hande; Mallikarjunan, Kumar; Kaaya, Archileo.

In: Food Analytical Methods, Vol. 9, No. 1, 01.01.2016, p. 105-113.

Research output: Contribution to journalArticle

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