Characterization of hamster recombinant monomorphic and polymorphic arylamine N-acetyltransferases. Bioactivation and mechanism-based inactivation studies with n-hydroxy-2-acetylaminofluorene

Kristina R.K. Sticha, Carl P. Bergstrom, Carston R. Wagner, Patrick E. Hanna

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17 Scopus citations

Abstract

The purified hamster recombinant arylamine N-acetyltransferases (NATs), rNAT1-9 and rNAT2-70D, were characterized for their capabilities to bioactivate N-hydroxy-2-acetylaminofluorene (N-OH-AAF) to DNA binding reactants and for their relative susceptibilities to mechanism-based inactivation by N-OH-AAF. The rate of DNA adduct formation resulting from rNAT1-9 bioactivation of [14C]N-OH-AAF was more than 30 times greater than that of rNAT2-70D-catalyzed bioactivation of [14C]N-OH-AAF. This result is consistent with substrate specificity data indicating that N-OH-AAF is a much better acetyl donor for hamster NAT1 than NAT2. Previous studies indicated that N-OH-AAF is a mechanism-based inactivator of hamster and rat NAT1. In the presence of N-OH-AAF, both rNAT1-9 and rNAT2-70D underwent irreversible, time-dependent inactivation that exhibited pseudo first-order kinetics and was saturable at higher N-OH-AAF concentrations. The enzymes were partially protected from inactivation by the presence of cofactor and substrates. The limiting rate constants (k(i)) and dissociation constants (K(I)) for inactivation by N-OH-AAF were determined. The second-order rate constants (k(i)/K(I)) were 22.1 min-1 mM-1 for rNAT1-9 and 1.0 min-1 mM-1 for rNAT2-70D, indicating that rNAT1-9 is approximately 20 times more susceptible than rNAT2-70D to inactivation by N-OH-AAF. The kinetic parameters for rNAT1-9 were nearly identical to values previously reported for partially purified hamster NAT1. Partition ratios were 504 for inactivation of rNAT1-9 by N-OH-AAF and 137 for inactivation of rNAT2-70D. Thus, a turnover of almost 4 times as many N-OH-AAF molecules is required to inactivate each molecule of rNAT1-9 than is needed to inactivate rNAT2-70D. The partition ratio data are consistent with the finding that rNAT1-9 catalyzes a higher rate of DNA adduct formation by N-OH-AAF than rNAT2-70D. The combined results indicate that the recombinant enzymes are catalytically and functionally identical to hamster NATs and, therefore, will be a useful resource for studies requiring purified NATs. Copyright (C) 1998 Elsevier Science, Inc.

Original languageEnglish (US)
Pages (from-to)47-59
Number of pages13
JournalBiochemical Pharmacology
Volume56
Issue number1
DOIs
StatePublished - Jul 1 1998

Bibliographical note

Funding Information:
This research was supported, in part, by National Cancer Institute Grant CA55334. We wish to thank Dr. Timothy W. Abraham for synthesizing 1,5-dimethyl-4-imidazole disulfide, Christine A. Sieg for constructing plasmid pPH9D, and Laurie Kleinbaum for her expert technical assistance. The authors are grateful to Professor M. W. Anders for calling their attention to the literature on ovothiols.

Keywords

  • Arylamine N-acetyltransferase
  • Bioactivation
  • Mechanism-based inactivation
  • N-hydroxy-2-acetylaminofluorene
  • Recombinant NATs

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