Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals

Klaudia Chrzastek; Dong hun Lee; Saad Gharaibeh; Aniko Zsak; Darrell R. Kapczynski

doi:10.1016/j.virol.2018.02.016

Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals

Klaudia Chrzastek, Dong hun Lee, Saad Gharaibeh, Aniko Zsak, Darrell R. Kapczynski

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Next-generation sequencing (NGS) technologies are a valuable tool to monitor changes in viral genomes and determine the genetic heterogeneity of viruses. In this study, NGS was applied to clinical poultry samples from Jordan to detect eleven H9N2 low pathogenic avian influenza viruses (LPAIV). All of the viruses tested belonged to Middle East A genetic group of G1 lineage. Deep sequencing demonstrated a high degree of heterogeneity of glutamine and leucine residues at position 226 in the hemagglutinin (HA) gene, which increases specificity to either avian or mammalian-type receptors. Moreover, additional amino acid changes in PB1, PA, M1, M2, and NS1 were identified among the viruses tested. Compared to single gene amplification, application of NGS for surveillance and characterization of H9N2 LPAIV provides a complete genetic profile of emerging isolates and better understanding of the potential of zoonotic transmissions to mammals.

Original language	English (US)
Pages (from-to)	195-201
Number of pages	7
Journal	Virology
Volume	518
DOIs	https://doi.org/10.1016/j.virol.2018.02.016
State	Published - May 2018

Bibliographical note

Funding Information:
This work was supported by USDA , ARS CRIS funds 6040–32000-062-00D and USDA-NIFA grant 60–6040-5-005 .

Publisher Copyright:
© 2018

Keywords

Avian influenza virus
H9N2
Hemagglutinin
Jordan
Mutation
Next generation sequencing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2018.02.016

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title = "Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals",

abstract = "Next-generation sequencing (NGS) technologies are a valuable tool to monitor changes in viral genomes and determine the genetic heterogeneity of viruses. In this study, NGS was applied to clinical poultry samples from Jordan to detect eleven H9N2 low pathogenic avian influenza viruses (LPAIV). All of the viruses tested belonged to Middle East A genetic group of G1 lineage. Deep sequencing demonstrated a high degree of heterogeneity of glutamine and leucine residues at position 226 in the hemagglutinin (HA) gene, which increases specificity to either avian or mammalian-type receptors. Moreover, additional amino acid changes in PB1, PA, M1, M2, and NS1 were identified among the viruses tested. Compared to single gene amplification, application of NGS for surveillance and characterization of H9N2 LPAIV provides a complete genetic profile of emerging isolates and better understanding of the potential of zoonotic transmissions to mammals.",

keywords = "Avian influenza virus, H9N2, Hemagglutinin, Jordan, Mutation, Next generation sequencing",

author = "Klaudia Chrzastek and Lee, {Dong hun} and Saad Gharaibeh and Aniko Zsak and Kapczynski, {Darrell R.}",

note = "Funding Information: This work was supported by USDA , ARS CRIS funds 6040–32000-062-00D and USDA-NIFA grant 60–6040-5-005 . Publisher Copyright: {\textcopyright} 2018",

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doi = "10.1016/j.virol.2018.02.016",

language = "English (US)",

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pages = "195--201",

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T1 - Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals

AU - Chrzastek, Klaudia

AU - Lee, Dong hun

AU - Gharaibeh, Saad

AU - Zsak, Aniko

AU - Kapczynski, Darrell R.

PY - 2018/5

Y1 - 2018/5

N2 - Next-generation sequencing (NGS) technologies are a valuable tool to monitor changes in viral genomes and determine the genetic heterogeneity of viruses. In this study, NGS was applied to clinical poultry samples from Jordan to detect eleven H9N2 low pathogenic avian influenza viruses (LPAIV). All of the viruses tested belonged to Middle East A genetic group of G1 lineage. Deep sequencing demonstrated a high degree of heterogeneity of glutamine and leucine residues at position 226 in the hemagglutinin (HA) gene, which increases specificity to either avian or mammalian-type receptors. Moreover, additional amino acid changes in PB1, PA, M1, M2, and NS1 were identified among the viruses tested. Compared to single gene amplification, application of NGS for surveillance and characterization of H9N2 LPAIV provides a complete genetic profile of emerging isolates and better understanding of the potential of zoonotic transmissions to mammals.

AB - Next-generation sequencing (NGS) technologies are a valuable tool to monitor changes in viral genomes and determine the genetic heterogeneity of viruses. In this study, NGS was applied to clinical poultry samples from Jordan to detect eleven H9N2 low pathogenic avian influenza viruses (LPAIV). All of the viruses tested belonged to Middle East A genetic group of G1 lineage. Deep sequencing demonstrated a high degree of heterogeneity of glutamine and leucine residues at position 226 in the hemagglutinin (HA) gene, which increases specificity to either avian or mammalian-type receptors. Moreover, additional amino acid changes in PB1, PA, M1, M2, and NS1 were identified among the viruses tested. Compared to single gene amplification, application of NGS for surveillance and characterization of H9N2 LPAIV provides a complete genetic profile of emerging isolates and better understanding of the potential of zoonotic transmissions to mammals.

KW - Avian influenza virus

KW - H9N2

KW - Hemagglutinin

KW - Jordan

KW - Mutation

KW - Next generation sequencing

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JO - Virology

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ER -

Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals

Abstract

Bibliographical note

Keywords

UN SDGs

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