Characterization of H9N2 avian influenza viruses from the Middle East demonstrates heterogeneity at amino acid position 226 in the hemagglutinin and potential for transmission to mammals

Klaudia Chrzastek, Dong hun Lee, Saad Gharaibeh, Aniko Zsak, Darrell R. Kapczynski

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Next-generation sequencing (NGS) technologies are a valuable tool to monitor changes in viral genomes and determine the genetic heterogeneity of viruses. In this study, NGS was applied to clinical poultry samples from Jordan to detect eleven H9N2 low pathogenic avian influenza viruses (LPAIV). All of the viruses tested belonged to Middle East A genetic group of G1 lineage. Deep sequencing demonstrated a high degree of heterogeneity of glutamine and leucine residues at position 226 in the hemagglutinin (HA) gene, which increases specificity to either avian or mammalian-type receptors. Moreover, additional amino acid changes in PB1, PA, M1, M2, and NS1 were identified among the viruses tested. Compared to single gene amplification, application of NGS for surveillance and characterization of H9N2 LPAIV provides a complete genetic profile of emerging isolates and better understanding of the potential of zoonotic transmissions to mammals.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalVirology
Volume518
DOIs
StatePublished - May 2018

Bibliographical note

Funding Information:
This work was supported by USDA , ARS CRIS funds 6040–32000-062-00D and USDA-NIFA grant 60–6040-5-005 .

Keywords

  • Avian influenza virus
  • H9N2
  • Hemagglutinin
  • Jordan
  • Mutation
  • Next generation sequencing

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