Characterization of Crude Oil Degrading Bacteria Isolated from Contaminated Soils Surrounding Gas Stations

Reda A.I. Abou-Shanab, Mohamed Eraky, Ahmed M. Haddad, Abdel Rahman B. Abdel-Gaffar, Ahmed M. Salem

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


A total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. Of the 20 isolates, RAM03, RAM06, RAM13, and RAM17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. These bacterial cultures had 16S rRNA gene sequences that were most similar to Ochrobactrum cytisi (RAM03), Ochrobactrum anthropi (RAM06 and RAM17), and Sinorhizobium meliloti (RAM13) with 96 %, 100 % and 99 %, and 99 % similarity. The tested strains revealed a promising potential for bioremediation of petroleum oil contamination as they could degrade >93 % and 54 % of total petroleum hydrocarbons (TPHs) in a liquid medium and soil amended with 4 % crude oil, respectively, after 30 day incubation. These bacteria could effectively remove both aliphatic and aromatic petroleum hydrocarbons. In conclusion, these strains could be considered as good prospects for their application in bioremediation of hydrocarbon contaminated environment.

Original languageEnglish (US)
Pages (from-to)684-688
Number of pages5
JournalBulletin of Environmental Contamination and Toxicology
Issue number5
StatePublished - Nov 1 2016

Bibliographical note

Publisher Copyright:
© 2016, Springer Science+Business Media New York.


  • 16S rRNA
  • Bacteria
  • Bioremediation
  • Contaminated soil
  • Petroleum hydrocarbons


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