Characterization of 'Candidatus Neoehrlichia lotoris' (family Anaplasmataceae) from raccoons (Procyon lotor)

Michael J. Yabsley, Staci M. Murphy, M. Page Luttrell, Benjamin R. Wilcox, Elizabeth W. Howerth, Ulrike G Munderloh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Recently, a novel ehrlichial organism was isolated from a raccoon (Procyon lotor) and the isolate (RAC413) was infectious to two naïve raccoons but not laboratory mice, rats or rabbits. In this study, amplification and sequencing of four gene targets (16S rRNA gene, groESL, gltA and rpoB) confirmed that the novel ehrlichial organism was a member of the family Anaplasmataceae and was most closely related to, but distinct from, 'Candidatus Neoehrlichia mikurensis' TK4456R and IS58. RAC413 shared the highest sequence similarity with members of the genus Ehrlichia (94.2-95.1, 80.9-83.1, 67.9-71.9 and 39.9-40.7% similarity for the 16S rRNA gene, groESL, gltA and rpoB, respectively). No sequence variation in three sequences (16S rRNA gene, groESL and gltA) was observed between the RAC413 isolate and five additional sequences amplified from blood of naturally infected raccoons from several geographically isolated populations in the south-eastern USA. Serum samples from four experimentally infected raccoons did not react to Ehrlichia canis, Ehrlichia chaffeensis, Anaplasma marginale or Anaplasma phagocytophilum antigens in an immunofluorescence assay or an Ehrlichia ewingii peptide in an ELISA format. On the basis of the distinctive molecular and serological characteristics and apparent host specificity of this ehrlichial organism, it is proposed that this organism be designated 'Candidatus Neoehrlichia lotoris' (reference strain RAC413R).

Original languageEnglish (US)
Pages (from-to)2794-2798
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume58
Issue number12
DOIs
StatePublished - Dec 1 2008

Fingerprint

Anaplasmataceae
Raccoons
Procyon lotor
rRNA Genes
Ehrlichia
gene
organisms
ribosomal RNA
Ehrlichia ewingii
Ehrlichia chaffeensis
genes
Ehrlichia canis
Anaplasma marginale
host specificity
Anaplasma phagocytophilum
isolated population
antigen
peptide
Host Specificity
serum

Cite this

Characterization of 'Candidatus Neoehrlichia lotoris' (family Anaplasmataceae) from raccoons (Procyon lotor). / Yabsley, Michael J.; Murphy, Staci M.; Luttrell, M. Page; Wilcox, Benjamin R.; Howerth, Elizabeth W.; Munderloh, Ulrike G.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 58, No. 12, 01.12.2008, p. 2794-2798.

Research output: Contribution to journalArticle

Yabsley, Michael J. ; Murphy, Staci M. ; Luttrell, M. Page ; Wilcox, Benjamin R. ; Howerth, Elizabeth W. ; Munderloh, Ulrike G. / Characterization of 'Candidatus Neoehrlichia lotoris' (family Anaplasmataceae) from raccoons (Procyon lotor). In: International Journal of Systematic and Evolutionary Microbiology. 2008 ; Vol. 58, No. 12. pp. 2794-2798.
@article{a004ee03d1dd4d5db39ead07cd4f1f66,
title = "Characterization of 'Candidatus Neoehrlichia lotoris' (family Anaplasmataceae) from raccoons (Procyon lotor)",
abstract = "Recently, a novel ehrlichial organism was isolated from a raccoon (Procyon lotor) and the isolate (RAC413) was infectious to two na{\"i}ve raccoons but not laboratory mice, rats or rabbits. In this study, amplification and sequencing of four gene targets (16S rRNA gene, groESL, gltA and rpoB) confirmed that the novel ehrlichial organism was a member of the family Anaplasmataceae and was most closely related to, but distinct from, 'Candidatus Neoehrlichia mikurensis' TK4456R and IS58. RAC413 shared the highest sequence similarity with members of the genus Ehrlichia (94.2-95.1, 80.9-83.1, 67.9-71.9 and 39.9-40.7{\%} similarity for the 16S rRNA gene, groESL, gltA and rpoB, respectively). No sequence variation in three sequences (16S rRNA gene, groESL and gltA) was observed between the RAC413 isolate and five additional sequences amplified from blood of naturally infected raccoons from several geographically isolated populations in the south-eastern USA. Serum samples from four experimentally infected raccoons did not react to Ehrlichia canis, Ehrlichia chaffeensis, Anaplasma marginale or Anaplasma phagocytophilum antigens in an immunofluorescence assay or an Ehrlichia ewingii peptide in an ELISA format. On the basis of the distinctive molecular and serological characteristics and apparent host specificity of this ehrlichial organism, it is proposed that this organism be designated 'Candidatus Neoehrlichia lotoris' (reference strain RAC413R).",
author = "Yabsley, {Michael J.} and Murphy, {Staci M.} and Luttrell, {M. Page} and Wilcox, {Benjamin R.} and Howerth, {Elizabeth W.} and Munderloh, {Ulrike G}",
year = "2008",
month = "12",
day = "1",
doi = "10.1099/ijs.0.65836-0",
language = "English (US)",
volume = "58",
pages = "2794--2798",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "12",

}

TY - JOUR

T1 - Characterization of 'Candidatus Neoehrlichia lotoris' (family Anaplasmataceae) from raccoons (Procyon lotor)

AU - Yabsley, Michael J.

AU - Murphy, Staci M.

AU - Luttrell, M. Page

AU - Wilcox, Benjamin R.

AU - Howerth, Elizabeth W.

AU - Munderloh, Ulrike G

PY - 2008/12/1

Y1 - 2008/12/1

N2 - Recently, a novel ehrlichial organism was isolated from a raccoon (Procyon lotor) and the isolate (RAC413) was infectious to two naïve raccoons but not laboratory mice, rats or rabbits. In this study, amplification and sequencing of four gene targets (16S rRNA gene, groESL, gltA and rpoB) confirmed that the novel ehrlichial organism was a member of the family Anaplasmataceae and was most closely related to, but distinct from, 'Candidatus Neoehrlichia mikurensis' TK4456R and IS58. RAC413 shared the highest sequence similarity with members of the genus Ehrlichia (94.2-95.1, 80.9-83.1, 67.9-71.9 and 39.9-40.7% similarity for the 16S rRNA gene, groESL, gltA and rpoB, respectively). No sequence variation in three sequences (16S rRNA gene, groESL and gltA) was observed between the RAC413 isolate and five additional sequences amplified from blood of naturally infected raccoons from several geographically isolated populations in the south-eastern USA. Serum samples from four experimentally infected raccoons did not react to Ehrlichia canis, Ehrlichia chaffeensis, Anaplasma marginale or Anaplasma phagocytophilum antigens in an immunofluorescence assay or an Ehrlichia ewingii peptide in an ELISA format. On the basis of the distinctive molecular and serological characteristics and apparent host specificity of this ehrlichial organism, it is proposed that this organism be designated 'Candidatus Neoehrlichia lotoris' (reference strain RAC413R).

AB - Recently, a novel ehrlichial organism was isolated from a raccoon (Procyon lotor) and the isolate (RAC413) was infectious to two naïve raccoons but not laboratory mice, rats or rabbits. In this study, amplification and sequencing of four gene targets (16S rRNA gene, groESL, gltA and rpoB) confirmed that the novel ehrlichial organism was a member of the family Anaplasmataceae and was most closely related to, but distinct from, 'Candidatus Neoehrlichia mikurensis' TK4456R and IS58. RAC413 shared the highest sequence similarity with members of the genus Ehrlichia (94.2-95.1, 80.9-83.1, 67.9-71.9 and 39.9-40.7% similarity for the 16S rRNA gene, groESL, gltA and rpoB, respectively). No sequence variation in three sequences (16S rRNA gene, groESL and gltA) was observed between the RAC413 isolate and five additional sequences amplified from blood of naturally infected raccoons from several geographically isolated populations in the south-eastern USA. Serum samples from four experimentally infected raccoons did not react to Ehrlichia canis, Ehrlichia chaffeensis, Anaplasma marginale or Anaplasma phagocytophilum antigens in an immunofluorescence assay or an Ehrlichia ewingii peptide in an ELISA format. On the basis of the distinctive molecular and serological characteristics and apparent host specificity of this ehrlichial organism, it is proposed that this organism be designated 'Candidatus Neoehrlichia lotoris' (reference strain RAC413R).

UR - http://www.scopus.com/inward/record.url?scp=58949093119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58949093119&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.65836-0

DO - 10.1099/ijs.0.65836-0

M3 - Article

VL - 58

SP - 2794

EP - 2798

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 12

ER -