Characterization of Campylobacter jejuni, Campylobacter upsaliensis, and a novel Campylobacter sp. in a captive non-human primate zoological collection

Jonathan B. Clayton, Jessica L. Danzeisen, Timothy J. Johnson, Ava M. Trent, Shivdeep S. Hayer, Tami Murphy, Arno Wuenschmann, Megan Elder, Zeli Shen, Anthony Mannion, Erin Bryant, Dan Knights, James G. Fox

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: The aim of this study was to longitudinally investigate the prevalence and characterization of Campylobacter spp. from non-human primates primate (NHP) with a history of endemic diarrhea housed at Como Park Zoo. Methods: Fecal samples from 33 symptom-free NHP belonging to eight different species were collected weekly for 9 weeks. Species-level characterization and phylogenetic analysis of isolates included biochemical testing and 16S rRNA sequencing. Results: Campylobacter spp. were isolated from the feces of 42% (14/33) of the primates. Three Campylobacter spp. (C upsaliensis, C jejuni, and novel Campylobacter sp.) were identified from three NHP species. A possible positive host Campylobacter species-specificity was observed. However, no statistical association was observed between the isolation of Campylobacter spp. and age and sex of the animal. Conclusions: The study revealed the value of conducting repeated fecal sampling to establish the overall prevalence of Campylobacter in zoo-maintained NHP; it also importantly identifies a novel Campylobacter sp. isolated from white-faced saki monkeys.

Original languageEnglish (US)
Pages (from-to)114-122
Number of pages9
JournalJournal of Medical Primatology
Volume48
Issue number2
DOIs
StatePublished - Apr 2019

Bibliographical note

Funding Information:
Funding information This research was funded by the University of Minnesota, the National Institutes of Health PharmacoNeuroImmunology Fellowship NIH/NIDA T32 DA007097-32 awarded to JC, and NIH grants P30-ES002109, R01 OD011141, and T32-OD010978 (all to JGF). The funders did not play any role in the study or in the preparation of the article or decision to publish. The authors would like to thank the Como Park Zoo Primate Keepers for their assistance with fecal collection, sample identification, and providing all background information necessary to conduct such a study. We thank the University of Minnesota College of Veterinary Medicine's Veterinary Diagnostic Laboratory (VDL) for providing information related to the diagnostic testing history at Como Zoo.

Funding Information:
This research was funded by the University of Minnesota, the National Institutes of Health PharmacoNeuroImmunology Fellowship NIH/NIDA T32 DA007097-32 awarded to JC, and NIH grants P30-ES002109, R01 OD011141, and T32-OD010978 (all to JGF). The funders did not play any role in the study or in the preparation of the article or decision to publish.

Publisher Copyright:
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.

Keywords

  • Campylobacter
  • captivity
  • diarrhea
  • gastrointestinal tract
  • non-human primate

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