Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5mM) whereas a few hygromycin B resistant isolates were also found. Copyright (C) 1999 Elsevier Science B.V.
Bibliographical noteFunding Information:
This work was supported in part by Dean's Fund, College of Veterinary Medicine, Manhattan, KA and the Kansas Agricultural Experiment Station, Manhattan, KA. We thank Ms. Ila Axton and Ms. Monika M. Walburger for excellent secretarial assistance and Ms. Michelle A. Swanson for help with propagation of BCV isolates in cell culture. Authors thank Ms. Cassie Clough and Ms. M. McCue for technical assistance. This article (Contribution No. 96-92-J) is from the Kansas Agricultural Experiment Station.
- Antigenic subtypes
- Bovine coronavirus
- Hygromycin B