Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer

B. Melichar, J. M. Hudson, T. Honda, T. Tuttle, J. L. Murray, J. T. Wharton, G. G. Loannides

Research output: Contribution to journalArticlepeer-review


We investigated the proliferation, expansion and recognition of cytotoxic lymphocytes (CTL) from breast tumors (BrCTL). Tumor infiltrating lymphocytes (TIL) isolated from solid tumors and malignant effusions from 15 patients expanded with interleukin-2 between 5-200 fold over 4 weeks as predominantly T cell lines. 6 of 15 TIL lines were predominantly CD8+ while 9 of 15 CTL line were predominantly CD4+. 3 of 5 HLA-A2+ CTL-TIL lines showed specific recognition of autologous tumor. 4 of 6 HLA-A2+ CTL lines recognized an immunodominant epitope of HER-2, 369-377. BrCTL recognized 10-14 peaks of peptides isolated from breast and ovarian tumors and separated by high performance liquid chromatography (HPLC) eluting with distinct retention times in two gradients of acetonitrile. Several peaks were common among freshly isolated tumors and established tumor lines. These results may be of interest for characterization of common tumor antigens and immunotherapy of breast cancer.

Original languageEnglish (US)
Pages (from-to)A1470
JournalFASEB Journal
Issue number6
StatePublished - 1996


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