Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer

B. Melichar; J. M. Hudson; T. Honda; T. Tuttle; J. L. Murray; J. T. Wharton; G. G. Loannides

Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer

B. Melichar, J. M. Hudson, T. Honda, T. Tuttle, J. L. Murray, J. T. Wharton, G. G. Loannides

Surgery

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the proliferation, expansion and recognition of cytotoxic lymphocytes (CTL) from breast tumors (BrCTL). Tumor infiltrating lymphocytes (TIL) isolated from solid tumors and malignant effusions from 15 patients expanded with interleukin-2 between 5-200 fold over 4 weeks as predominantly T cell lines. 6 of 15 TIL lines were predominantly CD8+ while 9 of 15 CTL line were predominantly CD4+. 3 of 5 HLA-A2+ CTL-TIL lines showed specific recognition of autologous tumor. 4 of 6 HLA-A2+ CTL lines recognized an immunodominant epitope of HER-2, 369-377. BrCTL recognized 10-14 peaks of peptides isolated from breast and ovarian tumors and separated by high performance liquid chromatography (HPLC) eluting with distinct retention times in two gradients of acetonitrile. Several peaks were common among freshly isolated tumors and established tumor lines. These results may be of interest for characterization of common tumor antigens and immunotherapy of breast cancer.

Original language	English (US)
Pages (from-to)	A1470
Journal	FASEB Journal
Volume	10
Issue number	6
State	Published - 1996

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title = "Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer",

abstract = "We investigated the proliferation, expansion and recognition of cytotoxic lymphocytes (CTL) from breast tumors (BrCTL). Tumor infiltrating lymphocytes (TIL) isolated from solid tumors and malignant effusions from 15 patients expanded with interleukin-2 between 5-200 fold over 4 weeks as predominantly T cell lines. 6 of 15 TIL lines were predominantly CD8+ while 9 of 15 CTL line were predominantly CD4+. 3 of 5 HLA-A2+ CTL-TIL lines showed specific recognition of autologous tumor. 4 of 6 HLA-A2+ CTL lines recognized an immunodominant epitope of HER-2, 369-377. BrCTL recognized 10-14 peaks of peptides isolated from breast and ovarian tumors and separated by high performance liquid chromatography (HPLC) eluting with distinct retention times in two gradients of acetonitrile. Several peaks were common among freshly isolated tumors and established tumor lines. These results may be of interest for characterization of common tumor antigens and immunotherapy of breast cancer.",

author = "B. Melichar and Hudson, {J. M.} and T. Honda and T. Tuttle and Murray, {J. L.} and Wharton, {J. T.} and Loannides, {G. G.}",

year = "1996",

language = "English (US)",

volume = "10",

pages = "A1470",

journal = "FASEB Journal",

issn = "0892-6638",

publisher = "John Wiley & Sons Inc.",

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TY - JOUR

T1 - Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer

AU - Melichar, B.

AU - Hudson, J. M.

AU - Honda, T.

AU - Tuttle, T.

AU - Murray, J. L.

AU - Wharton, J. T.

AU - Loannides, G. G.

PY - 1996

Y1 - 1996

N2 - We investigated the proliferation, expansion and recognition of cytotoxic lymphocytes (CTL) from breast tumors (BrCTL). Tumor infiltrating lymphocytes (TIL) isolated from solid tumors and malignant effusions from 15 patients expanded with interleukin-2 between 5-200 fold over 4 weeks as predominantly T cell lines. 6 of 15 TIL lines were predominantly CD8+ while 9 of 15 CTL line were predominantly CD4+. 3 of 5 HLA-A2+ CTL-TIL lines showed specific recognition of autologous tumor. 4 of 6 HLA-A2+ CTL lines recognized an immunodominant epitope of HER-2, 369-377. BrCTL recognized 10-14 peaks of peptides isolated from breast and ovarian tumors and separated by high performance liquid chromatography (HPLC) eluting with distinct retention times in two gradients of acetonitrile. Several peaks were common among freshly isolated tumors and established tumor lines. These results may be of interest for characterization of common tumor antigens and immunotherapy of breast cancer.

AB - We investigated the proliferation, expansion and recognition of cytotoxic lymphocytes (CTL) from breast tumors (BrCTL). Tumor infiltrating lymphocytes (TIL) isolated from solid tumors and malignant effusions from 15 patients expanded with interleukin-2 between 5-200 fold over 4 weeks as predominantly T cell lines. 6 of 15 TIL lines were predominantly CD8+ while 9 of 15 CTL line were predominantly CD4+. 3 of 5 HLA-A2+ CTL-TIL lines showed specific recognition of autologous tumor. 4 of 6 HLA-A2+ CTL lines recognized an immunodominant epitope of HER-2, 369-377. BrCTL recognized 10-14 peaks of peptides isolated from breast and ovarian tumors and separated by high performance liquid chromatography (HPLC) eluting with distinct retention times in two gradients of acetonitrile. Several peaks were common among freshly isolated tumors and established tumor lines. These results may be of interest for characterization of common tumor antigens and immunotherapy of breast cancer.

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VL - 10

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Characterization of antigen recognition in tumor infiltrating lymphocytes in breast cancer

Abstract

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