Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

Sook San Wong; Sun Woo Yoon; Mark Zanin; Min Suk Song; Christine Oshansky; Hassan Zaraket; Stephanie Sonnberg; Adam Rubrum; Patrick Seiler; Angela Ferguson; Scott Krauss; Carol Cardona; Richard J. Webby; Beate Crossley

doi:10.1016/j.virol.2014.07.048

Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

Sook San Wong, Sun Woo Yoon, Mark Zanin, Min Suk Song, Christine Oshansky, Hassan Zaraket, Stephanie Sonnberg, Adam Rubrum, Patrick Seiler, Angela Ferguson, Scott Krauss, Carol Cardona, Richard J. Webby, Beate Crossley

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.

Original language	English (US)
Pages (from-to)	72-80
Number of pages	9
Journal	Virology
Volume	468-470
DOIs	https://doi.org/10.1016/j.virol.2014.07.048
State	Published - Nov 2014

Bibliographical note

Funding Information:
We thank Jennifer DeBeauchamp, Jeri-Carol Crumpton, Ira Tigner Jr., and Demetrios Syndetos for excellent technical assistance. We would also like to thank Peter Vogel from the Veterinary Pathology Core facility and staff of the Animal Resource Center for animal care and husbandry assistance. This work was supported by the American Lebanese Syrian Associated Charities (ALSAC) and the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) Center of Excellence for Influenza Research and Surveillance ( HHSN266200700005C ).

Keywords

H4N2
Low pathogenic avian influenza
Multibasic cleavage site
Outbreak
Quail

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2014.07.048

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Wong, S. S., Yoon, S. W., Zanin, M., Song, M. S., Oshansky, C., Zaraket, H., Sonnberg, S., Rubrum, A., Seiler, P., Ferguson, A., Krauss, S., Cardona, C., Webby, R. J., & Crossley, B. (2014). Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site. Virology, 468-470, 72-80. https://doi.org/10.1016/j.virol.2014.07.048

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title = "Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site",

abstract = "The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.",

keywords = "H4N2, Low pathogenic avian influenza, Multibasic cleavage site, Outbreak, Quail",

author = "Wong, {Sook San} and Yoon, {Sun Woo} and Mark Zanin and Song, {Min Suk} and Christine Oshansky and Hassan Zaraket and Stephanie Sonnberg and Adam Rubrum and Patrick Seiler and Angela Ferguson and Scott Krauss and Carol Cardona and Webby, {Richard J.} and Beate Crossley",

note = "Funding Information: We thank Jennifer DeBeauchamp, Jeri-Carol Crumpton, Ira Tigner Jr., and Demetrios Syndetos for excellent technical assistance. We would also like to thank Peter Vogel from the Veterinary Pathology Core facility and staff of the Animal Resource Center for animal care and husbandry assistance. This work was supported by the American Lebanese Syrian Associated Charities (ALSAC) and the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) Center of Excellence for Influenza Research and Surveillance ( HHSN266200700005C ). ",

year = "2014",

month = nov,

doi = "10.1016/j.virol.2014.07.048",

language = "English (US)",

volume = "468-470",

pages = "72--80",

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T1 - Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

AU - Wong, Sook San

AU - Yoon, Sun Woo

AU - Zanin, Mark

AU - Song, Min Suk

AU - Oshansky, Christine

AU - Zaraket, Hassan

AU - Sonnberg, Stephanie

AU - Rubrum, Adam

AU - Seiler, Patrick

AU - Ferguson, Angela

AU - Krauss, Scott

AU - Cardona, Carol

AU - Webby, Richard J.

AU - Crossley, Beate

N1 - Funding Information: We thank Jennifer DeBeauchamp, Jeri-Carol Crumpton, Ira Tigner Jr., and Demetrios Syndetos for excellent technical assistance. We would also like to thank Peter Vogel from the Veterinary Pathology Core facility and staff of the Animal Resource Center for animal care and husbandry assistance. This work was supported by the American Lebanese Syrian Associated Charities (ALSAC) and the National Institutes of Health/National Institute of Allergy and Infectious Diseases (NIH/NIAID) Center of Excellence for Influenza Research and Surveillance ( HHSN266200700005C ).

PY - 2014/11

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N2 - The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.

AB - The cleavage motif in the hemagglutinin (HA) protein of highly pathogenic H5 and H7 subtypes of avian influenza viruses is characterized by a peptide insertion or a multibasic cleavage site (MBCS). Here, we isolated an H4N2 virus from quails (Quail/CA12) with two additional arginines in the HA cleavage site, PEK. RRTR/G, forming an MBCS-like motif. Quail/CA12 is a reassortant virus with the HA and neuraminidase (NA) gene most similar to a duck-isolated H4N2 virus, PD/CA06 with a monobasic HA cleavage site. Quail/CA12 required exogenous trypsin for efficient growth in culture and caused no clinical illness in infected chickens. Quail/CA12 had high binding preference for α2,6-linked sialic acids and showed higher replication and transmission ability in chickens and quails than PD/CA06. Although the H4N2 virus remained low pathogenic, these data suggests that the acquisition of MBCS in the field is not restricted to H5 or H7 subtypes.

KW - H4N2

KW - Low pathogenic avian influenza

KW - Multibasic cleavage site

KW - Outbreak

KW - Quail

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VL - 468-470

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JO - Virology

JF - Virology

ER -

Characterization of an H4N2 influenza virus from Quails with a multibasic motif in the hemagglutinin cleavage site

Abstract

Bibliographical note

Keywords

UN SDGs

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