Characterization of an ethanol-inducible promoter system in Catharanthus roseus hairy roots

Christie A.M. Peebles, Sue Gibson, Jacqueline V. Shanks, Ka Yiu San

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Efforts to engineer Catharanthus roseus hairy roots to produce commercially significant amounts of valuable compounds, such as the terpenoid indole alkaloids vinblastine and vincristine, require the development of tools to study the effects of overexpressing key metabolic and regulatory genes. The use of inducible promoters allows researchers to control the timing and level of expression of genes of interest. In addition, use of inducible promoters allows researchers to use a single transgenic line as both the control and experimental line, minimizing the problems associated with clonal variation. We have previously characterized the use of a glucocorticoid-inducible promoter system to study the effects of gene overexpression within the terpenoid indole alkaloid pathway on metabolite production. Here the feasibility of using an ethanol-inducible promoter within C. roseus hairy roots is reported. This ethanol-inducible promoter is highly sensitive to ethanol concentration with a concentration of 0.005% ethanol causing a 6-fold increase in CAT reporter activity after 24 h of induction. The ethanol-inducible CAT activity increased 24-fold over a 72-h induction period with 0.5% ethanol.

Original languageEnglish (US)
Pages (from-to)1258-1260
Number of pages3
JournalBiotechnology Progress
Volume23
Issue number5
DOIs
StatePublished - Sep 1 2007

Fingerprint

Catharanthus
Catharanthus roseus
Ethanol
ethanol
promoter regions
Secologanin Tryptamine Alkaloids
indole alkaloids
terpenoids
researchers
Research Personnel
vinblastine
clonal variation
vincristine
gene overexpression
Vinblastine
Vincristine
Regulator Genes
glucocorticoids
regulator genes
engineers

Cite this

Characterization of an ethanol-inducible promoter system in Catharanthus roseus hairy roots. / Peebles, Christie A.M.; Gibson, Sue; Shanks, Jacqueline V.; San, Ka Yiu.

In: Biotechnology Progress, Vol. 23, No. 5, 01.09.2007, p. 1258-1260.

Research output: Contribution to journalArticle

Peebles, Christie A.M. ; Gibson, Sue ; Shanks, Jacqueline V. ; San, Ka Yiu. / Characterization of an ethanol-inducible promoter system in Catharanthus roseus hairy roots. In: Biotechnology Progress. 2007 ; Vol. 23, No. 5. pp. 1258-1260.
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