Characterization of a zinc blotting technique: Evidence that a retroviral gag protein binds zinc

L. A. Schiff, M. L. Nibert, B. N. Fields

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

We have characterized a simple method that uses 65ZnCl2 to detect zinc-binding proteins that have been immobilized on nitrocellulose. Conditions have been identified that permit the detection of as little as 1 μg of some zinc-binding proteins. The specificity of the binding is indicated by the ability of other divalent metal ions to compete with 65 Zn(II) in this assay. We have used this technique to provide evidence that the nucleic acid-binding gag protein of retroviruses also binds zinc. This technique can be applied to biological mixtures of proteins and may be used in proteolytic mapping studies to identify protein fragments that have zinc-binding activity.

Original languageEnglish (US)
Pages (from-to)4195-4199
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number12
DOIs
StatePublished - 1988

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