TY - JOUR
T1 - Characterization of a staphylococcus aureus surface virulence factor that promotes resistance to oxidative killing and infectious endocarditis
AU - Malachowa, Natalia
AU - Kohler, Petra L.
AU - Schlievert, Patrick M.
AU - Chuang, Olivia N.
AU - Dunny, Gary M
AU - Kobayashi, Scott D.
AU - Miedzobrodzki, Jacek
AU - Bohach, Gregory A.
AU - Seo, Keun Seok
PY - 2011/1
Y1 - 2011/1
N2 - Staphylococcus aureus is a prominent human pathogen and a leading cause of community-and hospitalacquired bacterial infections worldwide. Herein, we describe the identification and characterization of the S. aureus 67.6-kDa hypothetical protein, named for the surface factor promoting resistance to oxidative killing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced S. aureus strains and homologous to the myosin cross-reactive antigen of Streptococcus pyogenes. Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of S. aureus Newman and RN4220. Comparative analysis of wild-type S. aureus and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the S. aureus sok deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections.
AB - Staphylococcus aureus is a prominent human pathogen and a leading cause of community-and hospitalacquired bacterial infections worldwide. Herein, we describe the identification and characterization of the S. aureus 67.6-kDa hypothetical protein, named for the surface factor promoting resistance to oxidative killing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced S. aureus strains and homologous to the myosin cross-reactive antigen of Streptococcus pyogenes. Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of S. aureus Newman and RN4220. Comparative analysis of wild-type S. aureus and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the S. aureus sok deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections.
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U2 - 10.1128/IAI.00736-10
DO - 10.1128/IAI.00736-10
M3 - Article
C2 - 20937760
AN - SCOPUS:78650867913
SN - 0019-9567
VL - 79
SP - 342
EP - 352
JO - Infection and Immunity
JF - Infection and Immunity
IS - 1
ER -
