Characterization of a staphylococcus aureus surface virulence factor that promotes resistance to oxidative killing and infectious endocarditis

Natalia Malachowa, Petra L. Kohler, Patrick M. Schlievert, Olivia N. Chuang, Gary M Dunny, Scott D. Kobayashi, Jacek Miedzobrodzki, Gregory A. Bohach, Keun Seok Seo

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Staphylococcus aureus is a prominent human pathogen and a leading cause of community-and hospitalacquired bacterial infections worldwide. Herein, we describe the identification and characterization of the S. aureus 67.6-kDa hypothetical protein, named for the surface factor promoting resistance to oxidative killing (SOK) in this study. Sequence analysis showed that the SOK gene is conserved in all sequenced S. aureus strains and homologous to the myosin cross-reactive antigen of Streptococcus pyogenes. Immunoblotting and immunofluorescence analysis showed that SOK was copurified with membrane fractions and was exposed on the surface of S. aureus Newman and RN4220. Comparative analysis of wild-type S. aureus and an isogenic deletion strain indicated that SOK contributes to both resistance to killing by human neutrophils and to oxidative stress. In addition, the S. aureus sok deletion strain showed dramatically reduced aortic valve vegetation and bacterial cell number in a rabbit endocarditis model. These results, plus the suspected role of the streptococcal homologue in certain diseases such as acute rheumatic fever, suggest that SOK plays an important role in cardiovascular and other staphylococcal infections.

Original languageEnglish (US)
Pages (from-to)342-352
Number of pages11
JournalInfection and immunity
Volume79
Issue number1
DOIs
StatePublished - Jan 2011

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