Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice

Michael D. Carrithers; Lisette M. Carrithers; Jan Czyzyk; Octavian Henegariu

doi:10.1016/j.jneuroim.2007.04.007

Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice

Michael D. Carrithers, Lisette M. Carrithers, Jan Czyzyk, Octavian Henegariu

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We here describe a novel CD4 T cell adoptive transfer model of severe experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. This FI cross developed severe disease characterized by extensive parenchymal spinal cord and brain periventricular white matter infiltrates. In contrast, B10.PL mice developed mild disease characterized by meningeal predominant infiltrates. As determined by cDNA microarray and quantitative real time PCR expression analysis, histologic and flow cytometry analysis of inflammatory infiltrates, and attenuation of disease in class I-deficient and CD8-depleted F1 mice; this severe disease phenotype appears to be regulated by CNS infiltration of CD8 T lymphocytes early in the disease course.

Original language	English (US)
Pages (from-to)	31-43
Number of pages	13
Journal	Journal of Neuroimmunology
Volume	187
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2007.04.007
State	Published - Jul 2007
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health, the National Multiple Sclerosis Society and the Dana and Bumpus Foundations (to M.D.C.). A portion of this work was presented in abstract form at the annual meeting of the American Neurological Association. Appendix A

Keywords

Adoptive transfer
CD8 T cells
EAE/MS
cDNA microarray

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10.1016/j.jneuroim.2007.04.007

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title = "Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice",

abstract = "We here describe a novel CD4 T cell adoptive transfer model of severe experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. This FI cross developed severe disease characterized by extensive parenchymal spinal cord and brain periventricular white matter infiltrates. In contrast, B10.PL mice developed mild disease characterized by meningeal predominant infiltrates. As determined by cDNA microarray and quantitative real time PCR expression analysis, histologic and flow cytometry analysis of inflammatory infiltrates, and attenuation of disease in class I-deficient and CD8-depleted F1 mice; this severe disease phenotype appears to be regulated by CNS infiltration of CD8 T lymphocytes early in the disease course.",

keywords = "Adoptive transfer, CD8 T cells, EAE/MS, cDNA microarray",

author = "Carrithers, {Michael D.} and Carrithers, {Lisette M.} and Jan Czyzyk and Octavian Henegariu",

note = "Funding Information: This work was supported by the National Institutes of Health, the National Multiple Sclerosis Society and the Dana and Bumpus Foundations (to M.D.C.). A portion of this work was presented in abstract form at the annual meeting of the American Neurological Association. Appendix A ",

year = "2007",

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doi = "10.1016/j.jneuroim.2007.04.007",

language = "English (US)",

volume = "187",

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journal = "Journal of Neuroimmunology",

issn = "0165-5728",

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T1 - Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice

AU - Carrithers, Michael D.

AU - Carrithers, Lisette M.

AU - Czyzyk, Jan

AU - Henegariu, Octavian

N1 - Funding Information: This work was supported by the National Institutes of Health, the National Multiple Sclerosis Society and the Dana and Bumpus Foundations (to M.D.C.). A portion of this work was presented in abstract form at the annual meeting of the American Neurological Association. Appendix A

PY - 2007/7

Y1 - 2007/7

N2 - We here describe a novel CD4 T cell adoptive transfer model of severe experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. This FI cross developed severe disease characterized by extensive parenchymal spinal cord and brain periventricular white matter infiltrates. In contrast, B10.PL mice developed mild disease characterized by meningeal predominant infiltrates. As determined by cDNA microarray and quantitative real time PCR expression analysis, histologic and flow cytometry analysis of inflammatory infiltrates, and attenuation of disease in class I-deficient and CD8-depleted F1 mice; this severe disease phenotype appears to be regulated by CNS infiltration of CD8 T lymphocytes early in the disease course.

AB - We here describe a novel CD4 T cell adoptive transfer model of severe experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice. This FI cross developed severe disease characterized by extensive parenchymal spinal cord and brain periventricular white matter infiltrates. In contrast, B10.PL mice developed mild disease characterized by meningeal predominant infiltrates. As determined by cDNA microarray and quantitative real time PCR expression analysis, histologic and flow cytometry analysis of inflammatory infiltrates, and attenuation of disease in class I-deficient and CD8-depleted F1 mice; this severe disease phenotype appears to be regulated by CNS infiltration of CD8 T lymphocytes early in the disease course.

KW - Adoptive transfer

KW - CD8 T cells

KW - EAE/MS

KW - cDNA microarray

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JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1-2

ER -

Characterization of a severe parenchymal phenotype of experimental autoimmune encephalomyelitis in (C57BL6xB10.PL)F1 mice

Abstract

Bibliographical note

Keywords

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