Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins

Paul M. Orwin, Donald Y.M. Leung, Timothy J. Tripp, Gregory A. Bohach, Cathleen A. Earhart, Douglas H Ohlendorf, Patrick M. Schlievert

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88 Scopus citations

Abstract

Staphylococcus aureus is an important human pathogen, causing a variety of diseases. Major virulence factors of this organism include staphylococcal enterotoxins (SEs) that cause food poisoning and toxic shock syndrome. Our study identified a novel enterotoxin-like protein that is a member of the new subfamily (group V) of pyrogenic toxin superantigens (PTSAgs) and examined its biochemical and immunobiological properties. The gene encoding the SE-like protein is directly 5′ of another recently identified PTSAg, SEK. The SE-like protein had a molecular weight of 26000 and an experimentally determined isoelectric point between 7.5 and 8.0. We demonstrated that the PTSAg had many of the biological activities associated with SEs, including superantigenicity, pyrogenicity, and ability to enhance endotoxin shock, but lacked both lethality in rabbits when administered in subcutaneous miniosmotic pumps and emetic activity in monkeys. Recombinant protein stimulated human CD4 and CD8 T cells in a T cell receptor variable region, β chain (TCRVβ) specific manner. T cells bearing TCRVβ 2, 5.1, and 21.3 were significantly stimulated.

Original languageEnglish (US)
Pages (from-to)14033-14040
Number of pages8
JournalBiochemistry
Volume41
Issue number47
DOIs
StatePublished - Nov 26 2002

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    Orwin, P. M., Leung, D. Y. M., Tripp, T. J., Bohach, G. A., Earhart, C. A., Ohlendorf, D. H., & Schlievert, P. M. (2002). Characterization of a novel staphylococcal enterotoxin-like superantigen, a member of the group V subfamily of pyrogenic toxins. Biochemistry, 41(47), 14033-14040. https://doi.org/10.1021/bi025977q