Characterization of a novel broad-host-range polyvalent Tequatrovirus bacteriophage infecting Salmonella, Shigella, and Escherichia coli

Jaap S. Bosma; Eric Latyshev; Olivia X. Ma; Eleanore G. Hansen; Estephany Cortes-Ortega; Vijay S. Reddy; Vincent Noireaux; Steven D. Bowden

doi:10.1007/s00705-025-06236-2

Characterization of a novel broad-host-range polyvalent Tequatrovirus bacteriophage infecting Salmonella, Shigella, and Escherichia coli

Jaap S. Bosma, Eric Latyshev, Olivia X. Ma, Eleanore G. Hansen, Estephany Cortes-Ortega, Vijay S. Reddy, Vincent Noireaux, Steven D. Bowden

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Abstract

We isolated and characterized the novel polyvalent T-even type bacteriophage vB_SenS_Jbel from wastewater using an enrichment of three different Salmonella strains. The vB_SenS_Jbel virions have prolate icosahedral capsids approximately 100 nm long and 80 nm wide. The genome consists of linear, double-stranded DNA that is 165,566 bp long. Analysis of the genome and structure of vB_SenS_Jbel indicates that it belongs to the genus Tequatrovirus of the family Straboviridae. This novel polyvalent phage can infect Escherichia coli and multiple Salmonella and Shigella species through its unique tail fiber structure.

Original language	English (US)
Article number	47
Journal	Archives of Virology
Volume	170
Issue number	3
DOIs	https://doi.org/10.1007/s00705-025-06236-2
State	Published - Mar 2025

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abstract = "We isolated and characterized the novel polyvalent T-even type bacteriophage vB_SenS_Jbel from wastewater using an enrichment of three different Salmonella strains. The vB_SenS_Jbel virions have prolate icosahedral capsids approximately 100 nm long and 80 nm wide. The genome consists of linear, double-stranded DNA that is 165,566 bp long. Analysis of the genome and structure of vB_SenS_Jbel indicates that it belongs to the genus Tequatrovirus of the family Straboviridae. This novel polyvalent phage can infect Escherichia coli and multiple Salmonella and Shigella species through its unique tail fiber structure.",

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AU - Latyshev, Eric

AU - Ma, Olivia X.

AU - Hansen, Eleanore G.

AU - Cortes-Ortega, Estephany

AU - Reddy, Vijay S.

AU - Noireaux, Vincent

AU - Bowden, Steven D.

N1 - Publisher Copyright: © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2025.

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Characterization of a novel broad-host-range polyvalent Tequatrovirus bacteriophage infecting Salmonella, Shigella, and Escherichia coli

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