Icosahedral virus-like particles were isolated from Petunia hybrida cuttings with interveinal chlorotic mottling. The virus was transmitted by mechanical inoculation from infected to healthy P. hybrida, and was found to contain a bipartite RNA genome of 7.6 and 3.8 kilobases. Full genomic sequence was obtained by high-throughput sequencing combined with RACE amplification of the 5′-termini of RNAs 1 and 2, and reverse- transcription PCR amplification of the 3′-termini with oligo-dT and sequence specific primers. Based on particle morphology, genome organization, and phylogenetic analyses, it was concluded that the new virus is a member of the genus Nepovirus, subgroup A. This new virus causing a leaf mottling disease of petunia was provisionally named Petunia chlorotic mottle virus (PCMoV).
|Original language||English (US)|
|Number of pages||5|
|State||Published - Jun 2017|
Bibliographical notePublisher Copyright:
© 2017, American Phytopathological Society. All rights reserved.
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Electron MicroscopyUniversity Imaging Centers
University Imaging Centers
Mark A Sanders (Program Director) & Guillermo Marques (Scientific Director)University Imaging Centers