Characterization of a new nepovirus causing a leaf mottling disease in Petunia hybrida

Sara Bratsch, Benham Lockhart, Dimitre Mollov

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Icosahedral virus-like particles were isolated from Petunia hybrida cuttings with interveinal chlorotic mottling. The virus was transmitted by mechanical inoculation from infected to healthy P. hybrida, and was found to contain a bipartite RNA genome of 7.6 and 3.8 kilobases. Full genomic sequence was obtained by high-throughput sequencing combined with RACE amplification of the 5′-termini of RNAs 1 and 2, and reverse- transcription PCR amplification of the 3′-termini with oligo-dT and sequence specific primers. Based on particle morphology, genome organization, and phylogenetic analyses, it was concluded that the new virus is a member of the genus Nepovirus, subgroup A. This new virus causing a leaf mottling disease of petunia was provisionally named Petunia chlorotic mottle virus (PCMoV).

Original languageEnglish (US)
Pages (from-to)1017-1021
Number of pages5
JournalPlant disease
Volume101
Issue number6
DOIs
StatePublished - Jun 2017

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