Characterization of a hollow fiber bioartificial liver device

Susan Fugett Abu-Absi, Gargi Seth, Ramanathan A. Narayanan, Kristine Groehler, Pamela Lai, Mary L. Anderson, Timothy Sielaff, Wei Shou Hu

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A three-compartment bioartificial liver (BAL) has been developed for potential treatment of fulminant hepatic failure. It has been shown previously that viability and liver-specific functions were maintained in laboratory-scale bioreactors of such design. In this study, the performance of hepatocytes in a clinical-scale bioartificial liver was verified by sustained specific production rates of albumin and urea, along with oxygen consumption rates for up to 56 h and liver-specific gene expression for up to 72 h. In addition, transmission of porcine endogenous retrovirus and other type C retroviral particles across the hollow fibers was not detected under both normal and extreme operating fluxes. These results demonstrate that the clinical-scale BAL performs at a level similar to the laboratory scale and that it offers a viral barrier against porcine retroviruses.

Original languageEnglish (US)
Pages (from-to)419-422
Number of pages4
JournalArtificial Organs
Volume29
Issue number5
DOIs
StatePublished - May 1 2005

Keywords

  • Bioartificial liver
  • Cultured hepatocytes
  • Liver-specific gene expression
  • Viral transmission

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