Characterization of α/β- and γ-gliadins in commercial varieties and breeding lines of durum wheat using MALDI-TOF and A-PAGE gels

Santiago Marín, Javier Gil-Humanes, Alberto Hernando, Francisco Barro

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the durum wheat lines to two groups. The utility of MALDI-TOF MS to determine relationships among genotypes and for identification of durum wheat accessions is discussed.

Original languageEnglish (US)
Pages (from-to)735-747
Number of pages13
JournalBiochemical Genetics
Volume49
Issue number11-12
DOIs
StatePublished - Dec 1 2011

Keywords

  • Cereal breeding
  • Durum wheat
  • Gliadins
  • Mass spectrometry

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