Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant

Sourav R. Hota; Soumesh K. Padhi; Avishek Pahari; Bikash K. Behera; Biswaranjan Panda; Sunil K. Mor; Vikash K. Singh; Sagar M. Goyal; Niranjana Sahoo

doi:10.1007/s12088-022-01047-4

Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant

Sourav R. Hota
, Soumesh K. Padhi
, Avishek Pahari
, Bikash K. Behera
, Biswaranjan Panda
, Sunil K. Mor
, Vikash K. Singh
, Sagar M. Goyal
, Niranjana Sahoo

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

UNLABELLED: This study reports a rare fatal case of Chromobacterium violeceum OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97% homology with C. violeceum. The bacterial genome composed of 64.87% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6')) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well.

SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-022-01047-4.

Original language	English (US)
Pages (from-to)	627-633
Number of pages	7
Journal	Indian Journal of Microbiology
Volume	62
Issue number	4
DOIs	https://doi.org/10.1007/s12088-022-01047-4
State	Published - Dec 2022

Bibliographical note

Funding Information:
This study was supported by the officers, Department of Forests and Environment, Government of Odisha working in the concerned area.

Publisher Copyright:
© 2022, Association of Microbiologists of India.

Keywords

Chromobacterium violaceum
Elephant
Whole genome sequencing
Zoonotic disease

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10.1007/s12088-022-01047-4

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@article{d576598aab8647cd990d2cfd5c330a10,

title = "Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT\_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant",

abstract = "UNLABELLED: This study reports a rare fatal case of Chromobacterium violeceum OUAT\_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97\% homology with C. violeceum. The bacterial genome composed of 64.87\% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6')) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-022-01047-4.",

keywords = "Chromobacterium violaceum, Elephant, Whole genome sequencing, Zoonotic disease",

author = "Hota, \{Sourav R.\} and Padhi, \{Soumesh K.\} and Avishek Pahari and Behera, \{Bikash K.\} and Biswaranjan Panda and Mor, \{Sunil K.\} and Singh, \{Vikash K.\} and Goyal, \{Sagar M.\} and Niranjana Sahoo",

note = "Funding Information: This study was supported by the officers, Department of Forests and Environment, Government of Odisha working in the concerned area. Publisher Copyright: {\textcopyright} 2022, Association of Microbiologists of India.",

year = "2022",

month = dec,

doi = "10.1007/s12088-022-01047-4",

language = "English (US)",

volume = "62",

pages = "627--633",

journal = "Indian Journal of Microbiology",

issn = "0046-8991",

publisher = "Springer",

number = "4",

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TY - JOUR

T1 - Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017

T2 - A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant

AU - Hota, Sourav R.

AU - Padhi, Soumesh K.

AU - Pahari, Avishek

AU - Behera, Bikash K.

AU - Panda, Biswaranjan

AU - Mor, Sunil K.

AU - Singh, Vikash K.

AU - Goyal, Sagar M.

AU - Sahoo, Niranjana

N1 - Funding Information: This study was supported by the officers, Department of Forests and Environment, Government of Odisha working in the concerned area. Publisher Copyright: © 2022, Association of Microbiologists of India.

PY - 2022/12

Y1 - 2022/12

N2 - UNLABELLED: This study reports a rare fatal case of Chromobacterium violeceum OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97% homology with C. violeceum. The bacterial genome composed of 64.87% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6')) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-022-01047-4.

AB - UNLABELLED: This study reports a rare fatal case of Chromobacterium violeceum OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97% homology with C. violeceum. The bacterial genome composed of 64.87% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6')) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-022-01047-4.

KW - Chromobacterium violaceum

KW - Elephant

KW - Whole genome sequencing

KW - Zoonotic disease

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UR - https://www.scopus.com/pages/publications/85140975376#tab=citedBy

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AN - SCOPUS:85140975376

SN - 0046-8991

VL - 62

SP - 627

EP - 633

JO - Indian Journal of Microbiology

JF - Indian Journal of Microbiology

IS - 4

ER -

Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant

Abstract

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