Characterization and RH mapping of bovine microsatellites generated from a microdissected BTA20-specific DNA library

G. H. Li; W. S. Liu; A. Takasuga; T. Watanabe; C. M. Carpio; A. Rink; Y. Sugimoto; F. A. Ponce De León; C. W. Beattie

doi:10.1111/j.1365-2052.2005.01258.x

Characterization and RH mapping of bovine microsatellites generated from a microdissected BTA20-specific DNA library

G. H. Li, W. S. Liu, A. Takasuga, T. Watanabe, C. M. Carpio, A. Rink, Y. Sugimoto, F. A. Ponce De León, C. W. Beattie

Animal Science

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Bovine chromosome 20 (BTA20) is associated with several quantitative trait loci (QTL) for meat tenderness, birth weight, milk yield and composition. Fine mapping of these QTL requires the development of additional informative markers to increase the resolution of the BTA20 genetic and physical maps. A BTA20-specific library was constructed by means of microdissection and microcloning, and screened for dinucleotide repeats with (CA)₁₆ and (GT)₁₆ oligos. A total of 60 new microsatellites (MS) were developed and characterized for polymorphism using the U.S. Department of Agriculture (USDA)/Meat Animal Research Center (MARC) bovine reference family, of which 53 markers were informative in this family. The number of alleles for these loci varied from 1 to 14. with an average of 6.5. Thirty-three of these MSs, together with 105 markers previously mapped to BTA20, were scored on a 7000-rad cattle-hamster whole-genome radiation hybrid panel (SUNbRH), resulting in a high-resolution RH_{7000 rad} map for BTA20.

Original language	English (US)
Pages (from-to)	146-151
Number of pages	6
Journal	Animal Genetics
Volume	36
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2052.2005.01258.x
State	Published - Apr 2005

Keywords

Bovine
Bovine chromosome 20
Microsatellites
Radiation hybrid map

Publisher link

10.1111/j.1365-2052.2005.01258.x

Find It

Find It at U of M Libraries

Cite this

@article{f55a1a678578420f9fdc639a9c79a3f3,

title = "Characterization and RH mapping of bovine microsatellites generated from a microdissected BTA20-specific DNA library",

abstract = "Bovine chromosome 20 (BTA20) is associated with several quantitative trait loci (QTL) for meat tenderness, birth weight, milk yield and composition. Fine mapping of these QTL requires the development of additional informative markers to increase the resolution of the BTA20 genetic and physical maps. A BTA20-specific library was constructed by means of microdissection and microcloning, and screened for dinucleotide repeats with (CA)16 and (GT)16 oligos. A total of 60 new microsatellites (MS) were developed and characterized for polymorphism using the U.S. Department of Agriculture (USDA)/Meat Animal Research Center (MARC) bovine reference family, of which 53 markers were informative in this family. The number of alleles for these loci varied from 1 to 14. with an average of 6.5. Thirty-three of these MSs, together with 105 markers previously mapped to BTA20, were scored on a 7000-rad cattle-hamster whole-genome radiation hybrid panel (SUNbRH), resulting in a high-resolution RH7000 rad map for BTA20.",

keywords = "Bovine, Bovine chromosome 20, Microsatellites, Radiation hybrid map",

author = "Li, {G. H.} and Liu, {W. S.} and A. Takasuga and T. Watanabe and Carpio, {C. M.} and A. Rink and Y. Sugimoto and {Ponce De Le{\'o}n}, {F. A.} and Beattie, {C. W.}",

year = "2005",

month = apr,

doi = "10.1111/j.1365-2052.2005.01258.x",

language = "English (US)",

volume = "36",

pages = "146--151",

journal = "Animal Genetics",

issn = "0268-9146",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "2",

}

TY - JOUR

T1 - Characterization and RH mapping of bovine microsatellites generated from a microdissected BTA20-specific DNA library

AU - Li, G. H.

AU - Liu, W. S.

AU - Takasuga, A.

AU - Watanabe, T.

AU - Carpio, C. M.

AU - Rink, A.

AU - Sugimoto, Y.

AU - Ponce De León, F. A.

AU - Beattie, C. W.

PY - 2005/4

Y1 - 2005/4

N2 - Bovine chromosome 20 (BTA20) is associated with several quantitative trait loci (QTL) for meat tenderness, birth weight, milk yield and composition. Fine mapping of these QTL requires the development of additional informative markers to increase the resolution of the BTA20 genetic and physical maps. A BTA20-specific library was constructed by means of microdissection and microcloning, and screened for dinucleotide repeats with (CA)16 and (GT)16 oligos. A total of 60 new microsatellites (MS) were developed and characterized for polymorphism using the U.S. Department of Agriculture (USDA)/Meat Animal Research Center (MARC) bovine reference family, of which 53 markers were informative in this family. The number of alleles for these loci varied from 1 to 14. with an average of 6.5. Thirty-three of these MSs, together with 105 markers previously mapped to BTA20, were scored on a 7000-rad cattle-hamster whole-genome radiation hybrid panel (SUNbRH), resulting in a high-resolution RH7000 rad map for BTA20.

AB - Bovine chromosome 20 (BTA20) is associated with several quantitative trait loci (QTL) for meat tenderness, birth weight, milk yield and composition. Fine mapping of these QTL requires the development of additional informative markers to increase the resolution of the BTA20 genetic and physical maps. A BTA20-specific library was constructed by means of microdissection and microcloning, and screened for dinucleotide repeats with (CA)16 and (GT)16 oligos. A total of 60 new microsatellites (MS) were developed and characterized for polymorphism using the U.S. Department of Agriculture (USDA)/Meat Animal Research Center (MARC) bovine reference family, of which 53 markers were informative in this family. The number of alleles for these loci varied from 1 to 14. with an average of 6.5. Thirty-three of these MSs, together with 105 markers previously mapped to BTA20, were scored on a 7000-rad cattle-hamster whole-genome radiation hybrid panel (SUNbRH), resulting in a high-resolution RH7000 rad map for BTA20.

KW - Bovine

KW - Bovine chromosome 20

KW - Microsatellites

KW - Radiation hybrid map

UR - http://www.scopus.com/inward/record.url?scp=17144394474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17144394474&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2052.2005.01258.x

DO - 10.1111/j.1365-2052.2005.01258.x

M3 - Article

C2 - 15771726

AN - SCOPUS:17144394474

SN - 0268-9146

VL - 36

SP - 146

EP - 151

JO - Animal Genetics

JF - Animal Genetics

IS - 2

ER -

Characterization and RH mapping of bovine microsatellites generated from a microdissected BTA20-specific DNA library

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this