Characterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila

Ted J. Brummel; Vern Twombly; Guillermo Marqués; Jeffrey L. Wrana; Stuart J. Newfeld; Liliana Attisano; Joan Massagué; Michael B. O'Connor; William M. Gelbart

doi:10.1016/0092-8674(94)90295-X

Characterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila

Ted J. Brummel, Vern Twombly, Guillermo Marqués, Jeffrey L. Wrana, Stuart J. Newfeld, Liliana Attisano, Joan Massagué, Michael B. O'Connor, William M. Gelbart

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

275 Scopus citations

Abstract

The dpp/BMP family of TGFβ-related factors controls numerous events in pattern formation and morphogenesis. How these polypeptide signals are received and transduced by target cells is largely unknown. We combine molecular and genetic approaches to establish that the Drosophila saxophone (sax) gene encodes a dpp receptor. We compare the structural properties and expression patterns of sax with a second dpp receptor encoded by the thick veins (tkv) gene. While the sax gene is expressed ubiquitously, tkv is expressed in a highly localized and dynamic pattern during development. Some, but not all, of the tkv expression pattern parallels that of dpp. Ubiquitous expression of a tkv transgene rescues both tkv and sax loss-of-function mutations. Thus, there is at least partial functional overlap of the sax and tkv receptors in vivo. We consider these observations in terms of possible ligand-receptor interactions during Drosophila development.

Original language	English (US)
Pages (from-to)	251-261
Number of pages	11
Journal	Cell
Volume	78
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(94)90295-X
State	Published - Jul 29 1994

Bibliographical note

Funding Information:
The first three authors have contributed equally to this work and should be considered co-first authors. We thank M. Hoffmann for wmmunica-tions prior to publication. We wish to thank T. Schilpbach. D. Gubb, S. Russo, S. Parks, C. Rushlow, and S. Roth for materials provided, useful discussions, or both. This work was supported by grants from the National Institutes of Health to J. M. and to Memorial Sloan-Kettering Cancer Center. J. L. W. and L. A. are Medical Research Council of Canada postdoctoral fellows. J. M. is a Howard Hughes Medical Institute Investigator. This work was also supported by an NIH grant to W. M. G. and to M. B. 0. V. T. was a National Research Service Award (NRSA) genetics trainee and S. N. is a NRSA poskloc-toral fellow. G. M. is a postdoctoral fellow of the United States-Spain Fulbright Program. Additionally, we thank J. Wozney for BMP-2.

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title = "Characterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila",

abstract = "The dpp/BMP family of TGFβ-related factors controls numerous events in pattern formation and morphogenesis. How these polypeptide signals are received and transduced by target cells is largely unknown. We combine molecular and genetic approaches to establish that the Drosophila saxophone (sax) gene encodes a dpp receptor. We compare the structural properties and expression patterns of sax with a second dpp receptor encoded by the thick veins (tkv) gene. While the sax gene is expressed ubiquitously, tkv is expressed in a highly localized and dynamic pattern during development. Some, but not all, of the tkv expression pattern parallels that of dpp. Ubiquitous expression of a tkv transgene rescues both tkv and sax loss-of-function mutations. Thus, there is at least partial functional overlap of the sax and tkv receptors in vivo. We consider these observations in terms of possible ligand-receptor interactions during Drosophila development.",

author = "Brummel, {Ted J.} and Vern Twombly and Guillermo Marqu{\'e}s and Wrana, {Jeffrey L.} and Newfeld, {Stuart J.} and Liliana Attisano and Joan Massagu{\'e} and O'Connor, {Michael B.} and Gelbart, {William M.}",

note = "Funding Information: The first three authors have contributed equally to this work and should be considered co-first authors. We thank M. Hoffmann for wmmunica-tions prior to publication. We wish to thank T. Schilpbach. D. Gubb, S. Russo, S. Parks, C. Rushlow, and S. Roth for materials provided, useful discussions, or both. This work was supported by grants from the National Institutes of Health to J. M. and to Memorial Sloan-Kettering Cancer Center. J. L. W. and L. A. are Medical Research Council of Canada postdoctoral fellows. J. M. is a Howard Hughes Medical Institute Investigator. This work was also supported by an NIH grant to W. M. G. and to M. B. 0. V. T. was a National Research Service Award (NRSA) genetics trainee and S. N. is a NRSA poskloc-toral fellow. G. M. is a postdoctoral fellow of the United States-Spain Fulbright Program. Additionally, we thank J. Wozney for BMP-2.",

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doi = "10.1016/0092-8674(94)90295-X",

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T1 - Characterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila

AU - Brummel, Ted J.

AU - Twombly, Vern

AU - Marqués, Guillermo

AU - Wrana, Jeffrey L.

AU - Newfeld, Stuart J.

AU - Attisano, Liliana

AU - Massagué, Joan

AU - O'Connor, Michael B.

AU - Gelbart, William M.

N1 - Funding Information: The first three authors have contributed equally to this work and should be considered co-first authors. We thank M. Hoffmann for wmmunica-tions prior to publication. We wish to thank T. Schilpbach. D. Gubb, S. Russo, S. Parks, C. Rushlow, and S. Roth for materials provided, useful discussions, or both. This work was supported by grants from the National Institutes of Health to J. M. and to Memorial Sloan-Kettering Cancer Center. J. L. W. and L. A. are Medical Research Council of Canada postdoctoral fellows. J. M. is a Howard Hughes Medical Institute Investigator. This work was also supported by an NIH grant to W. M. G. and to M. B. 0. V. T. was a National Research Service Award (NRSA) genetics trainee and S. N. is a NRSA poskloc-toral fellow. G. M. is a postdoctoral fellow of the United States-Spain Fulbright Program. Additionally, we thank J. Wozney for BMP-2.

PY - 1994/7/29

Y1 - 1994/7/29

N2 - The dpp/BMP family of TGFβ-related factors controls numerous events in pattern formation and morphogenesis. How these polypeptide signals are received and transduced by target cells is largely unknown. We combine molecular and genetic approaches to establish that the Drosophila saxophone (sax) gene encodes a dpp receptor. We compare the structural properties and expression patterns of sax with a second dpp receptor encoded by the thick veins (tkv) gene. While the sax gene is expressed ubiquitously, tkv is expressed in a highly localized and dynamic pattern during development. Some, but not all, of the tkv expression pattern parallels that of dpp. Ubiquitous expression of a tkv transgene rescues both tkv and sax loss-of-function mutations. Thus, there is at least partial functional overlap of the sax and tkv receptors in vivo. We consider these observations in terms of possible ligand-receptor interactions during Drosophila development.

AB - The dpp/BMP family of TGFβ-related factors controls numerous events in pattern formation and morphogenesis. How these polypeptide signals are received and transduced by target cells is largely unknown. We combine molecular and genetic approaches to establish that the Drosophila saxophone (sax) gene encodes a dpp receptor. We compare the structural properties and expression patterns of sax with a second dpp receptor encoded by the thick veins (tkv) gene. While the sax gene is expressed ubiquitously, tkv is expressed in a highly localized and dynamic pattern during development. Some, but not all, of the tkv expression pattern parallels that of dpp. Ubiquitous expression of a tkv transgene rescues both tkv and sax loss-of-function mutations. Thus, there is at least partial functional overlap of the sax and tkv receptors in vivo. We consider these observations in terms of possible ligand-receptor interactions during Drosophila development.

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Characterization and relationship of dpp receptors encoded by the saxophone and thick veins genes in Drosophila

Abstract

Bibliographical note

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