Characterization and immunopotentiating effects of the glycoprotein isolated from dioscorea batatas

Pham Thi Thu Huong, Chan Ho Lee, Mei Hong Li, Min Young Lee, Joon Ki Kim, Sun Mee Lee, Jong Heum Seon, Dong Cheol Lee, Young Jin Jeon

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We demonstrate that glycoprotein isolated from Dioscorea batatas (GDB) has immunostimulatory effects including macrophage activation. Analysis of infiltration of inflammatory cells into peritoneal cavity showed GDB treatment significantly increased the recruitment of macrophages, lymphocytes, neutrophils, and monocytes into the peritoneal cavity. Treatment of spleen cells isolated from C57BL/6 mice with GDB significantly increased the proliferation of B cells and T cells induced by LPS and ConA, respectively. Treatment with GDB significantly increased the cytolytic capacity of NK cells and macrophages against YAC-1 and B16 cells, respectively. In order to further confirm and investigate the mechanism of GDB on macrophage activation, we analyzed the effects of GDB on the cytokine expression including iNOS, IL-1β, and TNF-α in mouse macrophage cell line, RAW 264.7 cells. RT-PCR and ELISA showed that GDB increased the expression of IL-1β, and TNF-α, whereas iNOS was not induced by GDB. Collectively, this series of experiments indicates that GDB stimulates immune system including macrophage activation.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalKorean Journal of Physiology and Pharmacology
Issue number2
StatePublished - Apr 2011
Externally publishedYes


  • Glycoprotein
  • IL-1β
  • Macrophages
  • TNF-α


Dive into the research topics of 'Characterization and immunopotentiating effects of the glycoprotein isolated from dioscorea batatas'. Together they form a unique fingerprint.

Cite this