Characterization and distribution of angiotensin II binding sites in fetal and neonatal astrocytes from different rat brain regions

Serge P. Bottari, Nicholas Obermüller, Yvonne Bogdal, Kathleen R. Zahs, Christian F. Deschepper

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25 Scopus citations

Abstract

Although angiotensin II (Ang II) binding sites have been extensively investigated in brain, revealing the presence of both AT1 and AT2 subtypes in various areas, the question as to which cells express AT1 and AT2 sites is still open. We report here that primary cultures of astrocytes obtained from various brain regions of fetal (F17) and one-day-old rats express Ang II binding sites belonging only to the AT1 subtype. The binding sites have the same binding profile in all regions tested; however, much less binding was observed in membranes of astrocytes derived from cortical than from subcortical regions and almost none were found in neonatal cortex. In addition, the dispersion method used at the onset of culture affects the number of binding sites present at the end of the culture period.

Original languageEnglish (US)
Pages (from-to)372-376
Number of pages5
JournalBrain Research
Volume585
Issue number1-2
DOIs
StatePublished - Jul 10 1992

Bibliographical note

Funding Information:
We thank Dr. M. de Gasparo for his scientific input and Mrs. M. Mele for technicala ssistanceT. his work was supportedi n part by USPHS Grants HL38774 and HL29714( C.F.D.).

Keywords

  • Angiotensin II
  • Angiotensin II receptor
  • Astrocytes
  • CGP 42112A
  • DuP 753
  • Fetal brain
  • Neonatal brain
  • Radioligand binding

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