Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

Muhammad N. Tahir, Ben Lockhart, Samuel Grinstead, Dimitre Mollov

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus. Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus, family Tombusviridae.

Original languageEnglish (US)
Pages (from-to)1099-1102
Number of pages4
JournalArchives of Virology
Volume162
Issue number4
DOIs
StatePublished - Apr 1 2017

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