Abstract
A PCR based method was developed for the identification of ceftiofur resistance genes (blaCMY-2, blaTEM-1, and ampC) in swine bacterial pathogens. Using this method, the ceftiofur resistant (n=76) and susceptible (n=45) strains of Bordetella bronchiseptica, Salmonella spp., Escherichia coli, and Pasteurella multocida were screened for the presence of these three genes. The resistant genes were detected in 70% (blaTEM-1), 68% (blaCMY-2) and 45% (ampC) of the resistant isolates and in 18% (blaTEM-1), 27% (blaCMY-2), and 36% (ampC) of the susceptible isolates. Results obtained in the present study showed widespread distribution of these three resistance genes in ceftiofur-resistant swine pathogens. It was also observed that more pathogens are acquiring these resistance genes.
Original language | English (US) |
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Pages (from-to) | 139-141 |
Number of pages | 3 |
Journal | Veterinary Journal |
Volume | 187 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2011 |
Keywords
- AmpC
- Bla
- Bla
- Ceftiofur
- β-Lactamase