Changes in Select Redox Proteins of the Retinal Pigment Epithelium in Age-related Macular Degeneration

Alejandra Decanini, Curtis L. Nordgaard, Xiao Feng, Deborah A Ferrington, Timothy W. Olsen

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

Purpose: To examine changes of select reduction-oxidation (redox) sensitive proteins from human donor retinal pigment epithelium (RPE) at four stages of age-related macular degeneration (AMD). Design: Experimental study. Methods: Human donor eyes were obtained from the Minnesota Lions Eye Bank and graded using the Minnesota Grading System (MGS) into four stages that correspond to stages defined by the age-related eye disease study (AREDS). Protein content in RPE homogenates was measured using Western immunoblotting with protein-specific antibodies. Results: The content of several antioxidant enzymes and specific proteins that facilitate refolding or degradation of oxidatively damaged proteins increased significantly in MGS stage 3. These proteins are involved in the primary (copper-zinc superoxide dismutase [CuZnSOD], manganese superoxide dismutase [MnSOD], and catalase) and secondary (heat shock protein [HSP] 27, HSP 90, and proteasome) defense against oxidative damage. Additionally, the insulin pro-survival receptor exhibited disease-related upregulation. Conclusions: The pattern of protein changes identified in human donor tissue graded using the MGS support the role of oxidative mechanisms in the pathogenesis and progression of AMD. The MGS uses nearly identical clinical definitions and grading criteria of AMD that are used in the AREDS, so our results apply to clinical and epidemiologic studies using similar definitions. Results from our protein analysis of human donor tissue helps to explain altered oxidative stress regulation and cell-survival pathways that occur in progressive stages of AMD.

Original languageEnglish (US)
JournalAmerican journal of ophthalmology
Volume143
Issue number4
DOIs
StatePublished - Jan 1 2007

Fingerprint

Retinal Pigment Epithelium
Macular Degeneration
Oxidation-Reduction
Proteins
Eye Diseases
Superoxide Dismutase
Eye Banks
Tissue Donors
Protein Refolding
HSP27 Heat-Shock Proteins
Lions
HSP90 Heat-Shock Proteins
Proteasome Endopeptidase Complex
Catalase
Proteolysis
Zinc
Copper
Epidemiologic Studies
Cell Survival
Oxidative Stress

Cite this

Changes in Select Redox Proteins of the Retinal Pigment Epithelium in Age-related Macular Degeneration. / Decanini, Alejandra; Nordgaard, Curtis L.; Feng, Xiao; Ferrington, Deborah A; Olsen, Timothy W.

In: American journal of ophthalmology, Vol. 143, No. 4, 01.01.2007.

Research output: Contribution to journalArticle

Decanini, Alejandra ; Nordgaard, Curtis L. ; Feng, Xiao ; Ferrington, Deborah A ; Olsen, Timothy W. / Changes in Select Redox Proteins of the Retinal Pigment Epithelium in Age-related Macular Degeneration. In: American journal of ophthalmology. 2007 ; Vol. 143, No. 4.
@article{c6a4a9404b42416eae040a1326467bc9,
title = "Changes in Select Redox Proteins of the Retinal Pigment Epithelium in Age-related Macular Degeneration",
abstract = "Purpose: To examine changes of select reduction-oxidation (redox) sensitive proteins from human donor retinal pigment epithelium (RPE) at four stages of age-related macular degeneration (AMD). Design: Experimental study. Methods: Human donor eyes were obtained from the Minnesota Lions Eye Bank and graded using the Minnesota Grading System (MGS) into four stages that correspond to stages defined by the age-related eye disease study (AREDS). Protein content in RPE homogenates was measured using Western immunoblotting with protein-specific antibodies. Results: The content of several antioxidant enzymes and specific proteins that facilitate refolding or degradation of oxidatively damaged proteins increased significantly in MGS stage 3. These proteins are involved in the primary (copper-zinc superoxide dismutase [CuZnSOD], manganese superoxide dismutase [MnSOD], and catalase) and secondary (heat shock protein [HSP] 27, HSP 90, and proteasome) defense against oxidative damage. Additionally, the insulin pro-survival receptor exhibited disease-related upregulation. Conclusions: The pattern of protein changes identified in human donor tissue graded using the MGS support the role of oxidative mechanisms in the pathogenesis and progression of AMD. The MGS uses nearly identical clinical definitions and grading criteria of AMD that are used in the AREDS, so our results apply to clinical and epidemiologic studies using similar definitions. Results from our protein analysis of human donor tissue helps to explain altered oxidative stress regulation and cell-survival pathways that occur in progressive stages of AMD.",
author = "Alejandra Decanini and Nordgaard, {Curtis L.} and Xiao Feng and Ferrington, {Deborah A} and Olsen, {Timothy W.}",
year = "2007",
month = "1",
day = "1",
doi = "10.1016/j.ajo.2006.12.006",
language = "English (US)",
volume = "143",
journal = "American Journal of Ophthalmology",
issn = "0002-9394",
publisher = "Elsevier USA",
number = "4",

}

TY - JOUR

T1 - Changes in Select Redox Proteins of the Retinal Pigment Epithelium in Age-related Macular Degeneration

AU - Decanini, Alejandra

AU - Nordgaard, Curtis L.

AU - Feng, Xiao

AU - Ferrington, Deborah A

AU - Olsen, Timothy W.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Purpose: To examine changes of select reduction-oxidation (redox) sensitive proteins from human donor retinal pigment epithelium (RPE) at four stages of age-related macular degeneration (AMD). Design: Experimental study. Methods: Human donor eyes were obtained from the Minnesota Lions Eye Bank and graded using the Minnesota Grading System (MGS) into four stages that correspond to stages defined by the age-related eye disease study (AREDS). Protein content in RPE homogenates was measured using Western immunoblotting with protein-specific antibodies. Results: The content of several antioxidant enzymes and specific proteins that facilitate refolding or degradation of oxidatively damaged proteins increased significantly in MGS stage 3. These proteins are involved in the primary (copper-zinc superoxide dismutase [CuZnSOD], manganese superoxide dismutase [MnSOD], and catalase) and secondary (heat shock protein [HSP] 27, HSP 90, and proteasome) defense against oxidative damage. Additionally, the insulin pro-survival receptor exhibited disease-related upregulation. Conclusions: The pattern of protein changes identified in human donor tissue graded using the MGS support the role of oxidative mechanisms in the pathogenesis and progression of AMD. The MGS uses nearly identical clinical definitions and grading criteria of AMD that are used in the AREDS, so our results apply to clinical and epidemiologic studies using similar definitions. Results from our protein analysis of human donor tissue helps to explain altered oxidative stress regulation and cell-survival pathways that occur in progressive stages of AMD.

AB - Purpose: To examine changes of select reduction-oxidation (redox) sensitive proteins from human donor retinal pigment epithelium (RPE) at four stages of age-related macular degeneration (AMD). Design: Experimental study. Methods: Human donor eyes were obtained from the Minnesota Lions Eye Bank and graded using the Minnesota Grading System (MGS) into four stages that correspond to stages defined by the age-related eye disease study (AREDS). Protein content in RPE homogenates was measured using Western immunoblotting with protein-specific antibodies. Results: The content of several antioxidant enzymes and specific proteins that facilitate refolding or degradation of oxidatively damaged proteins increased significantly in MGS stage 3. These proteins are involved in the primary (copper-zinc superoxide dismutase [CuZnSOD], manganese superoxide dismutase [MnSOD], and catalase) and secondary (heat shock protein [HSP] 27, HSP 90, and proteasome) defense against oxidative damage. Additionally, the insulin pro-survival receptor exhibited disease-related upregulation. Conclusions: The pattern of protein changes identified in human donor tissue graded using the MGS support the role of oxidative mechanisms in the pathogenesis and progression of AMD. The MGS uses nearly identical clinical definitions and grading criteria of AMD that are used in the AREDS, so our results apply to clinical and epidemiologic studies using similar definitions. Results from our protein analysis of human donor tissue helps to explain altered oxidative stress regulation and cell-survival pathways that occur in progressive stages of AMD.

UR - http://www.scopus.com/inward/record.url?scp=33947383078&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947383078&partnerID=8YFLogxK

U2 - 10.1016/j.ajo.2006.12.006

DO - 10.1016/j.ajo.2006.12.006

M3 - Article

C2 - 17280640

AN - SCOPUS:33947383078

VL - 143

JO - American Journal of Ophthalmology

JF - American Journal of Ophthalmology

SN - 0002-9394

IS - 4

ER -