Changes in ribosomal protein rpL8 mRNA during the reproductive cycle of the mosquito, Aedes aegypti

Celeste A. Mazzacano, Ann M Fallon

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Changes in the abundance of ribosomal protein rpL8 mRNA were compared with 18S rRNA and vitellogenin mRNA in fat body from adult female Aedes aegypti during the reproductive cycle. Levels of rpL8 mRNA began to increase within 2 h after adult eclosion, peaked at about 24 h post-eclosion, and remained high throughout the next 48 h. During this same period, rRNA abundance increased about 2-fold. After the bloodmeal, levels of rpL8 mRNA gradually decreased over the next 48 h, while rRNA levels increased about 4- fold within 16-24 h post-bloodfeeding and eventually returned to previtellogenic levels. Vitellogenin mRNA was induced only after the bloodmeal, and disappeared by 48 h after feeding. After oviposition, rpL8 mRNA levels again increased to pre-bloodfeeding levels. These results indicate that rpL8 mRNA transcription in mosquito fat body is independent of rRNA transcription during the previtellogenic acquisition of competence and also during the post-bloodmeal ovarian cycle. Moreover, unlike the vitellogenin gene, the rpL8 gene is under post-transcriptional regulation in blood-fed females.

Original languageEnglish (US)
Pages (from-to)563-570
Number of pages8
JournalInsect Biochemistry and Molecular Biology
Volume26
Issue number6
DOIs
StatePublished - Jun 1996

Keywords

  • Developmental competence
  • Hormonal control
  • Ribosome
  • Vitellogenesis

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